Structure of PDB 2coj Chain B

Receptor sequence
>2cojB (length=358) Species: 9606 (Homo sapiens) [Search protein sequence]
TFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHI
KPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRS
AVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSL
GVKKPTKALLFVLLSPVGPYFFNPVSLWANPKYVRAWKGGTGDCKMGGNY
GSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELA
TPPLDGIILPGVTRRCILDLAHQWGEFKVSERYLTMDDLTTALEGNRVRE
MFGSGTACVVCPVSDILYKGETIHIPTMENGPKLASRILSKLTDIQYGRE
ERDWTIVL
3D structure
PDB2coj Structural determinants for branched-chain aminotransferase isozyme-specific inhibition by the anticonvulsant drug gabapentin
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) K222
Catalytic site (residue number reindexed from 1) K195
Enzyme Commision number 2.6.1.42: branched-chain-amino-acid transaminase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 GBN B Y90 V175 Y67 V152 MOAD: Ki=1.3mM
BS02 PLP B R119 R212 K222 Y227 E257 T260 L286 G288 V289 T290 T333 R96 R185 K195 Y200 E230 T233 L259 G261 V262 T263 T306
BS03 GBN B R163 T260 G332 T333 A334 R140 T233 G305 T306 A307 MOAD: Ki=1.3mM
Gene Ontology
Molecular Function
GO:0003824 catalytic activity
GO:0004084 branched-chain-amino-acid transaminase activity
GO:0008483 transaminase activity
GO:0052654 L-leucine-2-oxoglutarate transaminase activity
GO:0052655 L-valine-2-oxoglutarate transaminase activity
GO:0052656 L-isoleucine-2-oxoglutarate transaminase activity
Biological Process
GO:0000082 G1/S transition of mitotic cell cycle
GO:0006629 lipid metabolic process
GO:0008652 amino acid biosynthetic process
GO:0009081 branched-chain amino acid metabolic process
GO:0009082 branched-chain amino acid biosynthetic process
GO:0009098 L-leucine biosynthetic process
GO:0009099 L-valine biosynthetic process
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:2coj, PDBe:2coj, PDBj:2coj
PDBsum2coj
PubMed16141215
UniProtP54687|BCAT1_HUMAN Branched-chain-amino-acid aminotransferase, cytosolic (Gene Name=BCAT1)

[Back to BioLiP]