Structure of PDB 2cnb Chain B

Receptor sequence

>2cnbB (length=363) Species: 5691 (Trypanosoma brucei)

SHMRVLVCGGAGYIGSHFVRALLRDTNHSVVIVDSLVGTHGKSDHVETRE
NVARKLQQSDGPKPPWADRYAALEVGDVRNEDFLNGVFTRHGPIDAVVHM
CAFLAVGESVRDPLKYYDNNVVGILRLLQAMLLHKCDKIIFSSSAAIFGN
PTAEPIDINAKKSPESPYGESKLIAERMIRDCAEAYGIKGICLRYFNACG
AHEDGDIGEHYQGSTHLIPIILGRVMSDIADKRMPIFGTDYPTPDGTCVR
DYVHVCDLASAHILALDYVEKLGPNDKSKYFSVFNLGTSRGYSVREVIEV
ARKTTGHPIPVRECGRREGDPAYLVAASDKAREVLGWKPKYDTLEAIMET
SWKFQRTHPNGYA

3D structure

• PDB: 2cnb Trypanosoma Brucei Udp-Galactose-4-Epimerase in Ternary Complex with Nad+ and the Substrate Analogue Udp-4-Deoxy-4-Fluoro-Alpha-D-Galactose
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): S142 A143 A144 Y173 K177
• Catalytic site (residue number reindexed from 1): S144 A145 A146 Y168 K172
• Enzyme Commision number: 5.1.3.2: UDP-glucose 4-epimerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	UFG	B	L102 V104 S142 Y173 F201 N202 H221 L222 I225 M252 P253 I254 F255 C266 R268 V312 R335 D338	L104 V106 S144 Y168 F196 N197 H216 L217 I220 M234 P235 I236 F237 C248 R250 V294 R317 D320
BS02	NAD	B	G7 G10 Y11 I12 D32 S33 V35 G36 D75 M98 C99 A100 L102 N117 S140 Y173 K177 Y200 F201 A203	G9 G12 Y13 I14 D34 S35 V37 G38 D77 M100 C101 A102 L104 N119 S142 Y168 K172 Y195 F196 A198

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0003978 UDP-glucose 4-epimerase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0006012 galactose metabolic process
• GO:0033499 galactose catabolic process via UDP-galactose

Cellular Component

• GO:0005829 cytosol
• GO:0020015 glycosome

External links

• PDB: RCSB:2cnb, PDBe:2cnb, PDBj:2cnb
• PDBsum: 2cnb
• PubMed: 16946458
• UniProt: Q8T8E9