Structure of PDB 2cja Chain B

Receptor sequence
>2cjaB (length=489) Species: 269797 (Methanosarcina barkeri str. Fusaro) [Search protein sequence]
GSHMKLQFNLKAYFKTSADPTPAKDAIAALFEEANSTLLTRGAPEGQGAK
VTEWKLGEDRIELTLQSGRYVRVHDAIFRLRKQLAEALGKKYKIGIRGIE
VESFIIKVPADHELRMLKVPYIKSMENIEGGIQLELEVGEAEMKNRVPDR
ILTLLEEKIEAAQYGAKAEHWNLLWQREPMEHPFKEDPTQAMMKEGWLKR
GSSRGQWIHGPQSARIFRTFEKIVLEELLEPLGYREMIFPKLVTWEVWMK
SGHAKGVYPEIYYVCPPQTRDPDYWEEVADYYKVTHEVPTKLIKEKIAEP
IGGMCYAQCPPFWMYVAGETLPNEEIPVKVFDRSGTSHRYESGGIHGIER
VDEFHRIEIVWIGTKEEVLKCAEELHDRYMHIFNDILDIEWRKARVNTVG
TTDYEACLPYRGPDGEWLEFQNVSINGDKYPKGFNVKLQSGDELWSGCSG
VGLERWAAVFLAQKGLDPANWPEEFRNRVGEMPKGIRFL
3D structure
PDB2cja Structure of the Unusual Seryl-tRNA Synthetase Reveals a Distinct Zinc-Dependent Mode of Substrate Recognition
ChainB
Resolution2.2 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C306 R336 E338 R347 E355 D416 E432 N435 C461 R468
Catalytic site (residue number reindexed from 1) C309 R339 E341 R350 E358 D403 E419 N422 C448 R455
Enzyme Commision number 6.1.1.11: serine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 ZN B C306 E355 C461 C309 E358 C448
BS02 MG B R336 E338 R339 E341
BS03 MG B D416 E432 N435 D403 E419 N422
BS04 ATP B R336 E338 R347 V348 F351 R353 E432 G465 R468 R339 E341 R350 V351 F354 R356 E419 G452 R455
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004828 serine-tRNA ligase activity
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0046872 metal ion binding
Biological Process
GO:0006412 translation
GO:0006434 seryl-tRNA aminoacylation
GO:0016260 selenocysteine biosynthetic process
Cellular Component
GO:0005737 cytoplasm

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Cellular Component
External links
PDB RCSB:2cja, PDBe:2cja, PDBj:2cja
PDBsum2cja
PubMed16675947
UniProtQ46AN5|SYS2_METBF Type-2 serine--tRNA ligase (Gene Name=serS2)

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