Structure of PDB 2bn4 Chain B

Receptor sequence

>2bn4B (length=641) Species: 4932 (Saccharomyces cerevisiae)

NRDIAQVVTENNKNYLVLYASQTGTAEDYAKKFSKELVAKFNLNVMCADV
ENYDFESLNDVPVIVSIFISTYGEGDFPDGAVNFEDFICNAEAGALSNLR
YNMFGLGNSTYEFFNGAAKKAEKHLSAAGAIRLGKLGEADDGAGTTDEDY
MAWKDSILEVLKDELHLDEQEAKFTSQFQYTVLNEITDSMSLGEPSAHYL
PSHQLDGIQLGPFDLSQPYIAPIVKSRELFSSNDRNCIHSEFDLSGSNIK
YSTGDHLAVWPSNPLEKVEQFLSIFNLDPETIFDLKPLDPTVKVPFPTPT
TIGAAIKHYLEITGPVSRQLFSSLIQFAPNADVKEKLTLLSKDKDQFAVE
ITSKYFNIADALKYLSDGAKWDTVPMQFLVESVPQMTPRYYSISSSSLSE
KQTVHVTSIVENFPNPELPDAPPVVGVTTNLLRNIQLAQNNVNIAETNLP
VHYDLNGPRKLFANYKLPVHVRRSNFRLPSNPSTPVIMIGPGTGVAPFRG
FIRERVAFLESQKKGGNNVSLGKHILFYGSRNTDDFLYQDEWPEYAKKLD
GSFEMVVAHSRLPNTKKVYVQDKLKDYEDQVFEMINNGAFIYVCGDAKGM
AKGVSTALVGILSRGKSITTDEATELIKMLKTSGRYQEDVW

3D structure

• PDB: 2bn4 A Second Fmn-Binding Site in Yeast Nadph-Cytochrome P450 Reductase Suggests a Mechanism of Electron Transfer by Diflavin Reductases.
• Chain: B
• Resolution: 2.91 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y441 S442 C644 D689 W691
• Catalytic site (residue number reindexed from 1): Y391 S392 C594 D639 W641
• Enzyme Commision number: 1.6.2.4: NADPH--hemoprotein reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	H306 G364 P365 Y405 F406 R439 Y440 Y441 S442 I459 E461 V474 V475 G476 V477 T478 T479 T543 W691	H256 G314 P315 Y355 F356 R389 Y390 Y391 S392 I409 E411 V424 V425 G426 V427 T428 T429 T493 W641
BS02	FMN	B	S67 Q68 T69 T71 A72 S116 T117 Y118 N154 Y157 F159 F160 N161	S21 Q22 T23 T25 A26 S70 T71 Y72 N108 Y111 F113 F114 N115
BS03	NAP	B	I459 E461 P541 T543 S580 S610 R611 K617 Y619 Q621 D646 G649 M650	I409 E411 P491 T493 S530 S560 R561 K567 Y569 Q571 D596 G599 M600

Gene Ontology

Molecular Function

• GO:0003958 NADPH-hemoprotein reductase activity
• GO:0003959 NADPH dehydrogenase activity
• GO:0009055 electron transfer activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0050660 flavin adenine dinucleotide binding
• GO:0050661 NADP binding

Biological Process

• GO:0006696 ergosterol biosynthetic process
• GO:0016126 sterol biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005739 mitochondrion
• GO:0005741 mitochondrial outer membrane
• GO:0005783 endoplasmic reticulum
• GO:0005789 endoplasmic reticulum membrane
• GO:0005829 cytosol
• GO:0005886 plasma membrane

External links

• PDB: RCSB:2bn4, PDBe:2bn4, PDBj:2bn4
• PDBsum: 2bn4
• PubMed: 16407065
• UniProt: P16603|NCPR_YEAST NADPH--cytochrome P450 reductase (Gene Name=NCP1)