Structure of PDB 2bam Chain B

Receptor sequence
>2bamB (length=210) Species: 1390 (Bacillus amyloliquefaciens) [Search protein sequence]
MEVEKEFITDEAKELLSKDKLIQQAYNEVKTSICSPIWPATSKTFTINNT
EKNCNGVVPIKELCYTLLEDTYNWYREKPLDILKLEKKKGGPIDVYKEFI
ENSELKRVGMEFETGNISSAHRSMNKLLLGLKHGEIDLAIILMPIKQLAY
YLTDRVTNFEELEPYFELTEGQPFIFIGFNAEAYNSNVPLIPKGSDGMSK
RSIKKWKDKV
3D structure
PDB2bam The role of metals in catalysis by the restriction endonuclease BamHI.
ChainB
Resolution2.0 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B V57 K89 G90 G91 E113 T114 N116 R122 K126 T153 D154 R155 K193 V57 K89 G90 G91 E113 T114 N116 R122 K126 T153 D154 R155 K193
BS02 dna B N116 K146 R155 M198 N116 K146 R155 M198
Gene Ontology
Molecular Function
GO:0000287 magnesium ion binding
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:2bam, PDBe:2bam, PDBj:2bam
PDBsum2bam
PubMed9783752
UniProtP23940|T2BA_BACAM Type II restriction enzyme BamHI (Gene Name=bamHIR)

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