Structure of PDB 2aly Chain B

Receptor sequence

>2alyB (length=785) Species: 300852 (Thermus thermophilus HB8)

MRVPFSWLKAYVPELESPEVLEERLAGLGFETDRIERVFPIPRGVVFARV
LEAHPIPGTRLKRLVLDAGRTVEVVSGAENARKGIGVALALPGTELPGLG
QKVGERVIQGVRSFGMALSPRELGVGEYGGGLLEFPEDALPPGTPLSEAW
PEEVVLDLEVTPNRPDALGLLGLARDLHALGYALVEPEAALKAEALPLPF
ALKVEDPEGAPHFTLGYAFGLRVAPSPLWMQRALFAAGMRPINNVVDVTN
YVMLERAQPMHAFDLRFVGEGIAVRRAREGERLKTLDGVERTLHPEDLVI
AGWRGEESFPLGLAGVMGGAESEVREDTEAIALEVACFDPVSIRKTARRH
GLRTEASHRFERGVDPLGQVPAQRRALSLLQALAGARVAEALLEAGSPKP
PEAIPFRPEYANRLLGTSYPEAEQIAILKRLGCRVEGEGPTYRVTPPSHR
LDLRLEEDLVEEVARIQGYETIPLALPAFFPAPDNRGVEAPYRKEQRLRE
VLSGLGFQEVYTYSFMDPEDARRFRLDPPRLLLLNPLAPEKAALRTHLFP
GLVRVLKENLDLDRPERALLFEVGRVFREREETHLAGLLFGEGVGLPWAK
ERLSGYFLLKGYLEALFARLGLAFRVEAQAFPFLHPGVSGRVLVEGEEVG
FLGALHPEIAQELELPPVHLFELRLPLPDKPLAFQDPSRHPAAFRDLAVV
VPAPTPYGEVEALVREAAGPYLESLALFDLYQGPPLPEGHKSLAFHLRFR
HPKRTLRDEEVEEAVSRVAEALRARGFGLRGLDTP

3D structure

• PDB: 2aly Structural Basis for Discrimination of L-Phenylalanine from L-Tyrosine by Phenylalanyl-tRNA Synthetase
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Enzyme Commision number: 6.1.1.20: phenylalanine--tRNA ligase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MN	B	D452 E461	D452 E461

Gene Ontology

Molecular Function

• GO:0000049 tRNA binding
• GO:0000287 magnesium ion binding
• GO:0003723 RNA binding
• GO:0004812 aminoacyl-tRNA ligase activity
• GO:0004826 phenylalanine-tRNA ligase activity
• GO:0005524 ATP binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006412 translation
• GO:0006432 phenylalanyl-tRNA aminoacylation

Cellular Component

• GO:0005737 cytoplasm
• GO:0009328 phenylalanine-tRNA ligase complex

External links

• PDB: RCSB:2aly, PDBe:2aly, PDBj:2aly
• PDBsum: 2aly
• PubMed: 16338408
• UniProt: Q5SGX1|SYFB_THET8 Phenylalanine--tRNA ligase beta subunit (Gene Name=pheT)