Structure of PDB 2adm Chain B

Receptor sequence

>2admB (length=385) Species: 271 (Thermus aquaticus)

VETPPEVVDFMVSLAEAPRGGRVLEPACAHGPFLRAFREAHGTGYRFVGV
EIDPKALDLPPWAEGILADFLLWEPGEAFDLILGNPPYGIVGVFKAVKDL
YKKAFSTWKGKYNLYGAFLEKAVRLLKPGGVLVFVVPATWLVLEDFALLR
EFLAREGKTSVYYLGEVFPQKKVSAVVIRFQKSGKGLSLWDTQESESGFT
PILWAEYPHWEGEIIRFETEETRKLEISGMPLGDLFHIRFAARSPEFKKH
PAVRKEPGPGLVPVLTGRNLKPGWVDYEKNHSGLWMPKERAKELRDFYAT
PHLVVAHTKGTRVVAAWDERAYPWREEFHLLPKEGVRLDPSTLVQWLNSE
AMQKHVRTLYRDFVPHLTLRMLERLPVRREYGFHT

3D structure

• PDB: 2adm Differential binding of S-adenosylmethionine S-adenosylhomocysteine and Sinefungin to the adenine-specific DNA methyltransferase M.TaqI.
• Chain: B
• Resolution: 2.6 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): N105 P106 Y108 F196
• Catalytic site (residue number reindexed from 1): N85 P86 Y88 F168
• Enzyme Commision number: 2.1.1.72: site-specific DNA-methyltransferase (adenine-specific).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	B	A47 P52 E71 I72 D89 N105 P107	A27 P32 E51 I52 D69 N85 P87	PDBbind-CN: -logKd/Ki=5.70,Kd=2.0uM

Gene Ontology

Molecular Function

• GO:0003676 nucleic acid binding
• GO:0003677 DNA binding
• GO:0008168 methyltransferase activity
• GO:0009007 site-specific DNA-methyltransferase (adenine-specific) activity

Biological Process

• GO:0006304 DNA modification
• GO:0009307 DNA restriction-modification system
• GO:0032259 methylation

External links

• PDB: RCSB:2adm, PDBe:2adm, PDBj:2adm
• PDBsum: 2adm
• PubMed: 8995524
• UniProt: P14385|MTTA_THEAQ Type II methyltransferase M.TaqI (Gene Name=taqIM)