Structure of PDB 1zpt Chain B

Receptor sequence
>1zptB (length=285) Species: 562 (Escherichia coli) [Search protein sequence]
FFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNSIDRLSSLK
PKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
ARDYWNNGIRHIVALRGDLEMYASDLVTLLKEVADFDISVAAYPEVHPEA
KSAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIP
GILPVSNFKQAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMD
MVKILSREGVKDFHFYTLNRAEMSYAICHTLGVRP
3D structure
PDB1zpt Structures of NADH and CH(3)-H(4)Folate Complexes of Escherichia coli Methylenetetrahydrofolate Reductase Reveal a Spartan Strategy for a Ping-Pong Reaction
ChainB
Resolution1.95 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) S26 E28 D120 F223 H273
Catalytic site (residue number reindexed from 1) S24 E26 D118 F214 H264
Enzyme Commision number 1.5.1.54: methylenetetrahydrofolate reductase (NADH).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B T59 Y60 A62 H88 T90 L117 R118 G119 D120 Y131 A132 A150 Y152 H156 A159 D165 N168 K172 T57 Y58 A60 H86 T88 L115 R116 G117 D118 Y122 A123 A141 Y143 H147 A150 D156 N159 K163
BS02 NAI B E28 F30 T59 Q183 F184 F223 Y275 E26 F28 T57 Q174 F175 F214 Y266 MOAD: Ki=10uM
Gene Ontology
Molecular Function
GO:0004489 methylenetetrahydrofolate reductase (NAD(P)H) activity
GO:0016491 oxidoreductase activity
GO:0051087 protein-folding chaperone binding
GO:0071949 FAD binding
GO:0106312 methylenetetrahydrofolate reductase (NADH) activity
Biological Process
GO:0006555 methionine metabolic process
GO:0009086 methionine biosynthetic process
GO:0035999 tetrahydrofolate interconversion
Cellular Component
GO:0005829 cytosol
GO:0032991 protein-containing complex

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1zpt, PDBe:1zpt, PDBj:1zpt
PDBsum1zpt
PubMed16114881
UniProtP0AEZ1|METF_ECOLI 5,10-methylenetetrahydrofolate reductase (Gene Name=metF)

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