Structure of PDB 1zj8 Chain B

Receptor sequence

>1zj8B (length=546) Species: 83332 (Mycobacterium tuberculosis H37Rv) [Search protein sequence]

RNEGQWALGHREPLNANEELKKAGNPLDVRERIENIYAKQGFDSIDKTDL
RGRFRWWGLYTQREQGYDGTWTGDDNIDKLEAKYFMMRVRCDGGALSAAA
LRTLGQISTEFARDTADISDRQNVQYHWIEVENVPEIWRRLDDVGLQTTE
ACGDCPRVVLGSPLAGESLDEVLDPTWAIEEIVRRYIGKPDFADLPRKYK
TAISGLQDVAHEINDVAFIGVNHPEHGPGLDLWVGGGLSTNPMLAQRVGA
WVPLGEVPEVWAAVTSVFRDYGYRRLRAKARLKFLIKDWGIAKFREVLET
EYLKRPLIDGPAPEPVKHPIDHVGVQRLKNGLNAVGVAPIAGRVSGTILT
AVADLMARAGSDRIRFTPYQKLVILDIPDALLDDLIAGLDALGLQSRPSH
WRRNLMACSGIEFCKLSFAETRVRAQHLVPELERRLEDINSQLDVPITVN
INGCPNSCARIQIADIGFKGQMIDDGHGGSVEGFQVHLGGHLGLDAGFGR
KLRQHKVTSDELGDYIDRVVRNFVKHRSEGERFAQWVIRAEEDDLR

3D structure

PDB

1zj8 Siroheme- and [Fe4-S4]-dependent NirA from Mycobacterium tuberculosis Is a Sulfite Reductase with a Covalent Cys-Tyr Bond in the Active Site

Chain

Resolution

2.8 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	R97 R166 K207 K209 C417 C423 G462 C463 C467 A468
Catalytic site (residue number reindexed from 1)	R88 R157 K198 K200 C408 C414 G453 C454 C458 A459
Enzyme Commision number	1.8.7.1: assimilatory sulfite reductase (ferredoxin).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SF4	B	C417 C423 S426 G462 C463 N465 C467	C408 C414 S417 G453 C454 N456 C458
BS02	SRM	B	Y69 M95 R97 S128 D129 R130 N132 Q134 H136 R206 K207 K209 L247 S248 R290 Q379 S418 L425 R431 N465 C467 R469	Y60 M86 R88 S119 D120 R121 N123 Q125 H127 R197 K198 K200 L238 S239 R281 Q370 S409 L416 R422 N456 C458 R460

Gene Ontology

	Molecular Function
GO:0016002	sulfite reductase activity
GO:0016491	oxidoreductase activity
GO:0020037	heme binding
GO:0046872	metal ion binding
GO:0050311	sulfite reductase (ferredoxin) activity
GO:0051536	iron-sulfur cluster binding
GO:0051539	4 iron, 4 sulfur cluster binding

	Biological Process
GO:0000103	sulfate assimilation

	Cellular Component
GO:0005829	cytosol
GO:0009274	peptidoglycan-based cell wall

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1zj8, PDBe:1zj8, PDBj:1zj8
PDBsum	1zj8
PubMed	15917234
UniProt	P9WJ03\|SIR_MYCTU Sulfite reductase [ferredoxin] (Gene Name=sir)

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