Structure of PDB 1yfr Chain B

Receptor sequence
>1yfrB (length=448) Species: 63363 (Aquifex aeolicus) [Search protein sequence]
SLSAHEIRELFLSFFEKKGHTRVKSAPLVPENDPTLLFVNAGMVPFKNVF
LGLEKRPYKRATSCQKCLRVSGKHNDLEQVGYTSRHHTFFEMLGNFSFGD
YFKKEAIEYAWEFVTEVLKLPKEKLYVSVYKDDEEAYRIWNEHIGIPSER
IWRLGEEDNFWQMGDVGPCGPSSEIYVDRGEEYEGDERYLEIWNLVFMQY
NRDENGVLTPLPHPNIDTGMGLERIASVLQGKNSNFEIDIIFPLIQFGEE
VSGKKYGEKFETDVALRVIADHLRAITFAISDGVIPSNEGRGYVIRRILR
RAMRFGYKLGIENPFLYKGVDLVVDIMKEPYPELELSREFVKGIVKGEEK
RFIKTLKAGMEYIQEVIQKALEEGRKTLSGKEVFTAYDTYGFPVDLIDEI
AREKGLGIDLEGFQCELEEQRERARKHPVYSHLKELGKTSAFVGAAAL
3D structure
PDB1yfr Breaking sieve for steric exclusion of a noncognate amino acid from active site of a tRNA synthetase.
ChainB
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 6.1.1.7: alanine--tRNA ligase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B E174 N194 E174 N194
BS02 MG B E174 E191 E174 E191
BS03 ATP B R69 R85 H86 H87 F90 M92 E191 G221 R224 R69 R85 H86 H87 F90 M92 E191 G221 R224
Gene Ontology
Molecular Function
GO:0000166 nucleotide binding
GO:0003676 nucleic acid binding
GO:0004813 alanine-tRNA ligase activity
GO:0005524 ATP binding
Biological Process
GO:0006419 alanyl-tRNA aminoacylation
Cellular Component
GO:0005737 cytoplasm

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1yfr, PDBe:1yfr, PDBj:1yfr
PDBsum1yfr
PubMed15657145
UniProtO67323|SYA_AQUAE Alanine--tRNA ligase (Gene Name=alaS)

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