Structure of PDB 1yfi Chain B

Receptor sequence
>1yfiB (length=262) Species: 479 (Moraxella sp.) [Search protein sequence]
MRTELLSKLYDDFGIDQLPHTQHGVTSDRLGKLYEKYILDIFKDIESLKK
YNTNAFPQEKDISSKLLKALNLDLDNIIDVSSSDTDLGRTIAGGSPKTDA
TIRFTFHNQSSRLVPLNIKHSSKKKVSIAEYDVETICTGVGISDGELKEL
IRKHQNDQSAKLFTPVQKQRLTELLEPYRERFIRWCVTLRAEKSEGNILH
PDLLIRFQVIDREYVDVTIKNIDDYVSDRIAEGSKARKPGFGTGLNWTYA
SGSKAKKMQFKG
3D structure
PDB1yfi Two crystal forms of the restriction enzyme MspI-DNA complex show the same novel structure.
ChainB
Resolution2.7 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B K119 H120 S121 S122 K123 S127 G252 S253 K257 Q259 K261 K119 H120 S121 S122 K123 S127 G252 S253 K257 Q259 K261
BS02 dna B S159 A160 K161 N246 T248 Y249 S251 S159 A160 K161 N246 T248 Y249 S251
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1yfi, PDBe:1yfi, PDBj:1yfi
PDBsum1yfi
PubMed16195548
UniProtP11405|T2M1_MORSP Type II restriction enzyme MspI (Gene Name=mspIR)

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