Structure of PDB 1xzw Chain B

Receptor sequence

>1xzwB (length=426) Species: 4120 (Ipomoea batatas)

LPNAEDVDMPWDSDVFAVPSGYNAPQQVHITQGDYEGRGVIISWTTPYDK
AGANKVFYWSENSKSQKRAMGTVVTYKYYNYTSAFIHHCTIKDLEYDTKY
YYRLGFGDAKRQFWFVTPPKPGPDVPYVFGLIGDIGQTHDSNTTLTHYEQ
NSAKGQAVLFMGDLSYSNRWPNHDNNRWDTWGRFSERSVAYQPWIWTAGN
HEIDYAPDIGEYQPFVPFTNRYPTPHEASGSGDPLWYAIKRASAHIIVLS
SYSGFVKYSPQYKWFTSELEKVNRSETPWLIVLVHAPLYNSYEAHYMEGE
AMRAIFEPYFVYYKVDIVFSGHVHSYERSERVSNVAYNIVNAKCTPVSDE
SAPVYITIGDGGNSEGLASEMTQPQPSYSAFREASFGHGIFDIKNRTHAH
FSWHRNQDGASVEADSLWLLNRYWAS

3D structure

• PDB: 1xzw Phosphate forms an unusual tripodal complex with the Fe-Mn center of sweet potato purple acid phosphatase
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D634 D663 Y666 N700 H701 H785 A794 H795 H822 H824
• Catalytic site (residue number reindexed from 1): D134 D163 Y166 N200 H201 H285 A294 H295 H322 H324
• Enzyme Commision number: 3.1.3.2: acid phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FE	B	D634 D663 Y666 H824	D134 D163 Y166 H324
BS02	MN	B	D663 N700 H785 H822	D163 N200 H285 H322
BS03	PO4	B	D663 Y666 N700 H701 H822 H824 E865	D163 Y166 N200 H201 H322 H324 E365
BS04	PO4	B	R831 R922	R331 R422

Gene Ontology

Molecular Function

• GO:0003993 acid phosphatase activity
• GO:0016787 hydrolase activity
• GO:0046872 metal ion binding

Cellular Component

• GO:0005576 extracellular region

External links

• PDB: RCSB:1xzw, PDBe:1xzw, PDBj:1xzw
• PDBsum: 1xzw
• PubMed: 15625111
• UniProt: Q9SE00|PPAF1_IPOBA Purple acid phosphatase 1 (Gene Name=PAP1)