Structure of PDB 1xkv Chain B

Receptor sequence

>1xkvB (length=519) Species: 300852 (Thermus thermophilus HB8)

MQRLEALGIHPKKRVFWNTVSPVLVEHTLLRGEGLLAHHGPLVVDTTPYT
GRSPKDKFVVREPEVEGEIWWGEVNQPFAPEAFEALYQRVVQYLSERDLY
VQDLYAGADRRYRLAVRVVTESPWHALFARNMFILPRRFGNDDEVEAFVP
GFTVVHAPYFQAVPERDGTRSEVFVGISFQRRLVLIVGTKYAGEIKKSIF
TVMNYLMPKRGVFPMHASANVGKEGDVAVFFGLSGTGKTTLSTDPERPLI
GDDEHGWSEDGVFNFEGGCYAKVIRLSPEHEPLIYKASNQFEAILENVVV
NPESRRVQWDDDSKTENTRSSYPIAHLENVVESGVAGHPRAIFFLSADAY
GVLPPIARLSPEEAMYYFLSGYTARVPRATFSACFGAPFLPMHPGVYARM
LGEKIRKHAPRVYLVNTGWTGGPYGVGYRFPLPVTRALLKAALSGALENV
PYRRDPVFGFEVPLEAPGVPQELLNPRETWADKEAYDQQARKLARLFQEN
FQKYASGVAKEVAEAGPRT

3D structure

• PDB: 1xkv Structure of ATP-dependent phosphoenolpyruvate carboxykinase from Thermus thermophilus HB8 showing the structural basis of induced fit and thermostability.
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R52 K197 H216 S234 K238 T239 D253 R319
• Catalytic site (residue number reindexed from 1): R52 K197 H216 S234 K238 T239 D253 R319
• Enzyme Commision number: 4.1.1.49: phosphoenolpyruvate carboxykinase (ATP).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CA	B	R130 N131 F133 G267	R130 N131 F133 G267

Gene Ontology

Molecular Function

• GO:0004611 phosphoenolpyruvate carboxykinase activity
• GO:0004612 phosphoenolpyruvate carboxykinase (ATP) activity
• GO:0005524 ATP binding
• GO:0016831 carboxy-lyase activity
• GO:0017076 purine nucleotide binding
• GO:0046872 metal ion binding

Biological Process

• GO:0006094 gluconeogenesis

Cellular Component

• GO:0005737 cytoplasm
• GO:0005829 cytosol

External links

• PDB: RCSB:1xkv, PDBe:1xkv, PDBj:1xkv
• PDBsum: 1xkv
• PubMed: 16239727
• UniProt: Q5SLL5|PCKA_THET8 Phosphoenolpyruvate carboxykinase (ATP) (Gene Name=pckA)