Structure of PDB 1xjj Chain B

Receptor sequence

>1xjjB (length=611) Species: 2336 (Thermotoga maritima)

MKLSDLISRWIDVEPSKNAQIILRDRYFMKDLDGNYLETKWEDVARRVAR
VVATAELLNPSYKKNEKLDRIKEWEDIFFRVLKARLFIPNSPTLFNAGLG
VKHDLLWKPIDQMTLEDYEEIYRSRNHLHMLSACFVVPVGDSIEEIFEAV
KEYALITKVGGGVGSNFSELRPKGSFVAGTHGKASGPVSFMHVFNSAISV
VKQALMGILNINHPDIEEFIDAKKEAVLNFFNLSVGFPMDKKEILKLYEE
DGELELSHPRSTIRKKVKIRELFRKIATNAWKSGDPGLAFLGEMNKYYPL
YPHRKINSTNPCGEIGLSDYEACNLGSIDVAKFYNNGFVDLEALQELVQI
AVRFLDNVIDVNVFPIDKITKAVKESRRLGLGIMGFADLLYKLEIPYNSQ
EARDFAANLMAFIALHAHRTSYELGKEKGNFPLLEISRYRTEDNFVPFAM
GMSNYDDEIREVMKMTKEFRRNVALLTIAPTGSISNIADTSSGLEPNFLL
AYTRFYVNQVLREKLNPEILKRIEKELIEKGSLKDIPDVPEKIKKVFVVA
LDIDPMDHLLMQDAFQRYVDNNISKTINMPQSATVDDVLNVYLEALRTNV
RGITVYRDGSL

3D structure

• PDB: 1xjj Structural Mechanism of Allosteric Substrate Specificity Regulation in a Ribonucleotide Reductase
• Chain: B
• Resolution: 1.86 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C134 N320 C322 E324 C333 T626 V627
• Catalytic site (residue number reindexed from 1): C134 N310 C312 E314 C323 T604 V605
• Enzyme Commision number: 1.17.4.1: ribonucleoside-diphosphate reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	DGT	B	K158 V201	K158 V201
BS02	DGT	B	D141 S142 I143 R171 V177 S185 F190	D141 S142 I143 R171 V177 S185 F190
BS03	DGT	B	R26 S91 E324 P490 T491 G492 S493 R514	R26 S91 E314 P480 T481 G482 S483 R504

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
• GO:0005524 ATP binding
• GO:0031419 cobalamin binding

Biological Process

• GO:0009263 deoxyribonucleotide biosynthetic process

External links

• PDB: RCSB:1xjj, PDBe:1xjj, PDBj:1xjj
• PDBsum: 1xjj
• PubMed: 15475969
• UniProt: O33839