Structure of PDB 1xfc Chain B

Receptor sequence

>1xfcB (length=356) Species: 1773 (Mycobacterium tuberculosis)

LAEAMVDLGAIEHNVRVLREHAGHAQLMAVVKADGYGHGATRVAQTALGA
GAAELGVATVDEALALRADGITAPVLAWLHPPGIDFGPALLADVQVAVSS
LRQLDELLHAVRRTGRTATVTVKVDTGLNRNGVGPAQFPAMLTALRQAMA
EDAVRLRGLMSHMVYADKPDDSINDVQAQRFTAFLAQAREQGVRFEVAHL
SNSSATMARPDLTFDLVRPGIAVYGLSPVPALGDMGLVPAMTVKCAVALV
KSIRDTNLALLPIGYADGVFRSLGGRLEVLINGRRCPGVGRICMDQFMVD
LGPGPLDVAEGDEAILFGPGIRGEPTAQDWADLVGTIHYEVVTSPRGRIT
RTYREA

3D structure

• PDB: 1xfc The 1.9 A crystal structure of alanine racemase from Mycobacterium tuberculosis contains a conserved entryway into the active site.
• Chain: B
• Resolution: 1.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K42 R140 H172 R228 C318 D320
• Catalytic site (residue number reindexed from 1): K32 R130 H162 R218 C293 D295
• Enzyme Commision number: 5.1.1.1: alanine racemase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	K42 Y46 W88 H172 S213 R228 G230 I231 Y364	K32 Y36 W78 H162 S203 R218 G220 I221 Y339

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0005515 protein binding
• GO:0008784 alanine racemase activity
• GO:0016853 isomerase activity
• GO:0030170 pyridoxal phosphate binding

Biological Process

• GO:0006522 alanine metabolic process
• GO:0009252 peptidoglycan biosynthetic process
• GO:0030632 D-alanine biosynthetic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1xfc, PDBe:1xfc, PDBj:1xfc
• PDBsum: 1xfc
• PubMed: 15683232
• UniProt: P9WQA9|ALR_MYCTU Alanine racemase (Gene Name=alr)