Structure of PDB 1wcq Chain B

Receptor sequence
>1wcqB (length=601) Species: 1881 (Micromonospora viridifaciens) [Search protein sequence]
GEPLYTEQDLAVNGREGFPNYRIPALTVTPDGDLLASYDGRPTGIDAPGP
NSILQRRSTDGGRTWGEQQVVSAGQTTAPIKGFSDPSYLVDRETGTIFNF
HVYSQRQGFAGSRPGTDPADPNVLHANVATSTDGGLTWSHRTITADITPD
PGWRSRFAASGEGIQLRYGPHAGRLIQQYTIINAAGAFQAVSVYSDDHGR
TWRAGEAVGVGMDENKTVELSDGRVLLNSRDSARSGYRKVAVSTDGGHSY
GPVTIDRDLPDPTNNASIIRAFPDAPAGSARAKVLLFSNAASQTSRSQGT
IRMSCDDGQTWPVSKVFQPGSMSFSTLTALPDGTYGLLYEPGTGIRYANF
NLAWLGGICAPFTIPDVALEPGQQVTVPVAVTNQSGIAVPKPSLQLDASP
DWQVQGSVEPLMPGRQAKGQVTITVPAGTTPGRYRVGATLRTSAGNASTT
FTVTVGLLDQARMSIADVDSEETAREDGRASNVIDGNPSTFWHTEWSRAD
APGYPHRISLDLGGTHTISGLQYTRRQNSANEQVADYEIYTSLNGTTWDG
PVASGRFTTSLAPQRAVFPARDARYIRLVALSEQTGHKYAAVAELEVEGQ
R
3D structure
PDB1wcq Two Nucleophilic Mutants of the Micromonospora Viridifaciens Sialidase Operate with Retention of Configuration by Two Different Mechanisms.
ChainB
Resolution2.1 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D92 E260 F370
Catalytic site (residue number reindexed from 1) D46 E214 F324
Enzyme Commision number 3.2.1.18: exo-alpha-sialidase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 DAN B R68 I69 D92 D131 F203 D259 R276 R342 F370 R22 I23 D46 D85 F157 D213 R230 R296 F324 MOAD: Ki=0.55uM
Gene Ontology
Molecular Function
GO:0004308 exo-alpha-sialidase activity
GO:0016798 hydrolase activity, acting on glycosyl bonds
Biological Process
GO:0005975 carbohydrate metabolic process
GO:0006689 ganglioside catabolic process
GO:0009313 oligosaccharide catabolic process
Cellular Component
GO:0005576 extracellular region
GO:0005737 cytoplasm
GO:0016020 membrane
GO:0043231 intracellular membrane-bounded organelle

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1wcq, PDBe:1wcq, PDBj:1wcq
PDBsum1wcq
PubMed16206228
UniProtQ02834|NANH_MICVI Sialidase (Gene Name=nedA)

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