Structure of PDB 1w25 Chain B

Receptor sequence

>1w25B (length=454) Species: 190650 (Caulobacter vibrioides CB15)

SARILVVDDIEANVRLLEAKLTAEYYEVSTAMDGPTALAMAARDLPDIIL
LDVMMPGMDGFTVCRKLKDDPTTRHIPVVLITALDGRGDRIQGLESGASD
FLTKPIDDVMLFARVRSLTRFKLVIDELRQREASGRRMGVIAGAAARLDG
LGGRVLIVDDNERQAQRVAAELGVEHRPVIESDPEKAKISAGGPVDLVIV
NAAAKNFDGLRFTAALRSEERTRQLPVLAMVDPDDRGRMVKALEIGVNDI
LSRPIDPQELSARVKTQIQRKRYTDYLRNNLDHSLELAVTDQLTGLHNRR
YMTGQLDSLVKRATLGGDPVSALLIDIDFFKKINDTFGHDIGDEVLREFA
LRLASNVRAIDLPCRYGGEEFVVIMPDTALADALRIAERIRMHVSGSPFT
VAHGREMLNVTISIGVSATAGEGDTPEALLKRADEGVYQAKASGRNAVVG
KAAH

3D structure

• PDB: 1w25 Structural Basis of Activity and Allosteric Control of Diguanylate Cyclase
• Chain: B
• Resolution: 2.7 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.65: diguanylate cyclase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	MG	B	D9 D10 D53 M55	D8 D9 D52 M54	Manual survey: Ki=0.5uM (15569936)
BS02	C2E	B	R178 R359 D362 D383 I387 R390	R177 R358 D361 D382 I386 R389	Manual survey: Ki=0.5uM (15569936)
BS03	C2E	B	R148 V175 R178 S356 R359 A360 R390	R147 V174 R177 S355 R358 A359 R389	Manual survey: Ki=0.5uM (15569936)

Gene Ontology

Molecular Function

• GO:0005525 GTP binding
• GO:0016740 transferase activity
• GO:0046872 metal ion binding
• GO:0052621 diguanylate cyclase activity

Biological Process

• GO:0000160 phosphorelay signal transduction system
• GO:0030154 cell differentiation
• GO:0043709 cell adhesion involved in single-species biofilm formation
• GO:1902201 negative regulation of bacterial-type flagellum-dependent cell motility

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:1w25, PDBe:1w25, PDBj:1w25
• PDBsum: 1w25
• PubMed: 15569936
• UniProt: B8GZM2|PLED_CAUVN Response regulator PleD (Gene Name=pleD)