Structure of PDB 1w21 Chain B

Receptor sequence

>1w21B (length=389) Species: 11320 (Influenza A virus)

RTFLNLTKPLCEVNSWHILSKDNAIRIGEDAHILVTREPYLSCDPQGCRM
FALSQGTTLRGRHANGTIHDRSPFRALISWEMGQAPSPYNTRVECIGWSS
TSCHDGMSRMSICMSGPNNNASAVVWYGGRPITEIPSWAGNILRTQESEC
VCHKGVCPVVMTDGPANNRAATKIIYFKEGKIQKIEELAGNAQHIEECSC
YGAGGVIKCICRDNWKGANRPVITIDPEMMTHTSKYLCSKVLTDTSRPND
PTNGNCDAPITGGSPDPGVKGFAFLDGENSWLGRTISKDSRSGYEMLKVP
NAETDIQSGPISNQVIVNNQNWSGYSGAFIDYWANKECFNPCFYVELIRG
RPKESSVLWTSNSIVALCGSKKRLGSWSWHDGAEIIYFE

3D structure

• PDB: 1w21 The Crystal Structure of Type a Influenza Virus Neuraminidase of the N6 Subtype Reveals the Existence of Two Separate Neu5Ac Binding Sites
• Chain: B
• Resolution: 2.08 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R1299 Y1412
• Catalytic site (residue number reindexed from 1): R212 Y325
• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SIA	B	E1125 D1157 R1158 W1185 A1253 E1283 E1284 R1299 R1378 Y1412	E38 D70 R71 W98 A166 E196 E197 R212 R291 Y325
BS02	SIA	B	S1374 S1377 S1379 N1406 N1408 W1409	S287 S290 S292 N319 N321 W322
BS03	BMA	B	I1398 S1399	I311 S312
BS04	MAN	B	P1397 N1400	P310 N313
BS05	MAN	B	D1337 R1371	D250 R284
BS06	MAN	B	E1382 N1400	E295 N313
BS07	CA	B	D1300 G1304 D1331 P1354	D213 G217 D244 P267

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0005975 carbohydrate metabolic process

Cellular Component

• GO:0016020 membrane
• GO:0033644 host cell membrane
• GO:0055036 virion membrane

External links

• PDB: RCSB:1w21, PDBe:1w21, PDBj:1w21
• PDBsum: 1w21
• UniProt: Q6XV27|NRAM_I56A2 Neuraminidase (Gene Name=NA)