Structure of PDB 1w1j Chain B

Receptor sequence

>1w1jB (length=550) Species: 69488 (Penicillium simplicissimum) [Search protein sequence]

EFRPLTLPPKLSLSDFNEFIQDIIRIVGSENVEVISVDGSYMKPTHTHDP
HHVMDQDYFLASAIVAPRNVADVQSIVGLANKFSFPLWPISIGRNSGYGG
AAPRVSGSVVLDMGKNMNRVLEVNVEGAYCVVEPGVTYHDLHNYLEANNL
RDKLWLDVPDLGGGSVLGNAVERGVGYTPYGDHWMMHSGMEVVLANGELL
RTGMGALPDPKRPETMGLKPEDQPWSKIAHLFPYGFGPYIDGLFSQSNMG
IVTKIGIWLMPNPRGYQSYLITLPKDGDLKQAVDIIRPLRLGMALQNVPT
IRHILLDAAVLGDKRSYSSRTEPLSDEELDKIAKQLNLGRWNFYGALYGP
EPIRRVLWETIKDAFSAIPGVKFYFPEDTPENSVLRVRDKTMQGIPTYDE
LKWIDWLPNGAHLFFSPIAKVSGEDAMMQYAVTKKRCQEAGLDFIGTFTV
GMREMHHIVCIVFNKKDLIQKRKVQWLMRTLIDDCAANGWGEYRSHLAFM
DQIMETYNWNNSSFLRFNEVLKNAVDPNGIIAPGKSGVWPSQYSHVTWKL

3D structure

PDB

1w1j Laboratory-Evolved Vanillyl-Alcohol Oxidase Produces Natural Vanillin

Chain

Resolution

2.7 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	Y108 D170 E182 D192 D317 V397 E410 I414 H422 H466 Y503 R504 K545
Catalytic site (residue number reindexed from 1)	Y98 D160 E172 D182 D307 V387 E400 I404 H412 H456 Y493 R494 K535
Enzyme Commision number	1.1.3.38: vanillyl-alcohol oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	P99 S101 I102 G103 R104 N105 P169 D170 L171 G174 S175 N179 E182 V185 Y187 I261 V262 H422 F424 R504	P89 S91 I92 G93 R94 N95 P159 D160 L161 G164 S165 N169 E172 V175 Y177 I251 V252 H412 F414 R494
BS02	EUG	B	Y108 D170 V185 F424 H466 I468 Y503	Y98 D160 V175 F414 H456 I458 Y493

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004458	D-lactate dehydrogenase (cytochrome) activity
GO:0008720	D-lactate dehydrogenase activity
GO:0016491	oxidoreductase activity
GO:0018465	vanillyl-alcohol oxidase activity
GO:0050660	flavin adenine dinucleotide binding
GO:0071949	FAD binding

	Biological Process
GO:0015945	methanol metabolic process
GO:1903457	lactate catabolic process

	Cellular Component
GO:0005737	cytoplasm
GO:0005739	mitochondrion
GO:0005777	peroxisome

View graph for
Molecular Function

View graph for
Biological Process

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Cellular Component

External links

PDB	RCSB:1w1j, PDBe:1w1j, PDBj:1w1j
PDBsum	1w1j
PubMed	15169773
UniProt	P56216\|VAOX_PENSI Vanillyl-alcohol oxidase (Gene Name=VAOA)

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