Structure of PDB 1vrq Chain B

Receptor sequence

>1vrqB (length=402) Species: 31944 (Corynebacterium sp. U-96)

ADLLPEHPEFLWNNPEPKKSYDVVIVGGGGHGLATAYYLAKNHGITNVAV
LEKGWLAGGNMARNTTIIRSNYLWDESAGIYEKSLKLWEELPEELEYDFL
FSQRGVLNLAHTLGDVRESIRRVEANKFNGVDAEWLTPEQVKEVCPIINT
GDNIRYPVMGATYQPRAGIAKHDHVAWAFARKANEMGVDIIQNCEVTGFL
KDGEKVTGVKTTRGTILAGKVALAGAGHSSVLAELAGFELPIQSHPLQAL
VSELFEPVHPTVVMSNHIHVYVSQAHKGELVMGAGIDSYNGYGQRGAFHV
IEEQMAAAVELFPIFARAHVLRTWGGIVDTTMDASPIISKTPIQNLYVNC
GWGTGGFKGTPGAGYTLAHTIAHDEPHKLNAPFALERFETGHLIDEHGAA
AV

3D structure

• PDB: 1vrq Crystal structure of heterotetrameric sarcosine oxidase from Corynebacterium sp. U-96
• Chain: B
• Resolution: 2.2 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A62 T65 T66 G326 K358
• Catalytic site (residue number reindexed from 1): A62 T65 T66 G326 K358
• Enzyme Commision number: 1.5.3.24: sarcosine oxidase (5,10-methylenetetrahydrofolate-forming).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	V26 G27 G29 G30 H31 L51 E52 K53 G59 N60 M61 R63 N64 T65 I67 V196 A224 G225 H228 L247 G353 T354 G355 G356 F357 K358	V26 G27 G29 G30 H31 L51 E52 K53 G59 N60 M61 R63 N64 T65 I67 V196 A224 G225 H228 L247 G353 T354 G355 G356 F357 K358
BS02	FMN	B	A62 R63 H172 V251 E279 R322 W324	A62 R63 H172 V251 E279 R322 W324
BS03	DMG	B	T65 I67 R69 K358 A401	T65 I67 R69 K358 A401

Gene Ontology

Molecular Function

• GO:0000166 nucleotide binding
• GO:0008115 sarcosine oxidase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0046653 tetrahydrofolate metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1vrq, PDBe:1vrq, PDBj:1vrq
• PDBsum: 1vrq
• PubMed: 15946648
• UniProt: Q50LF2|TSOXB_CORS9 Sarcosine oxidase subunit beta (Gene Name=soxB)