Structure of PDB 1v3m Chain B

Receptor sequence

>1v3mB (length=686) Species: 1410 (Bacillus sp. 1011) [Search protein sequence]

APDTSVSNKQNFSTDVIYQIFTDRFSDGNPANNPTGAAFDGSCTNLRLYC
GGDWQGIINKINDGYLTGMGITAIWISQPVENIYSVINYSGVNNTAYHGY
WARDFKKTNPAYGTMQDFKNLIDTAHAHNIKVIIDFAPNHTSPASSDDPS
FAENGRLYDNGNLLGGYTNDTQNLFHHYGGTDFSTIENGIYKNLYDLADL
NHNNSSVDVYLKDAIKMWLDLGVDGIRVDAVKHMPFGWQKSFMATINNYK
PVFTFGEWFLGVNEISPEYHQFANESGMSLLDYRFAQKARQVFRDNTDNM
YGLKAMLEGSEVDYAQVNDQVTFIDNHDMERFHTSNGDRRKLEQALAFTL
TSRGVPAIYYGSEQYMSGGNDPDNRARLPSFSTTTTAYQVIQKLAPLRKS
NPAIAYGSTHERWINNDVIIYERKFGNNVAVVAINRNMNTPASITGLVTS
LPRGSYNDVLGGILNGNTLTVGAGGAASNFTLAPGGTAVWQYTTDATTPI
IGNVGPMMAKPGVTITIDGRGFGSGKGTVYFGTTAVTGADIVAWEDTQIQ
VKIPAVPGGIYDIRVANAAGAASNIYDNFEVLTGDQVTVRFVINNATTAL
GQNVFLTGNVSELGNWDPNNAIGPMYNQVVYQYPTWYYDVSVPAGQTIEF
KFLKKQGSTVTWEGGANRTFTTPTSGTATVNVNWQP

3D structure

PDB

1v3m Role of Phe283 in enzymatic reaction of cyclodextrin glycosyltransferase from alkalophilic Bacillus sp.1011: Substrate binding and arrangement of the catalytic site

Chain

Resolution

2.0 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D135 R227 D229 E257 H327 D328
Catalytic site (residue number reindexed from 1)	D135 R227 D229 E257 H327 D328
Enzyme Commision number	2.4.1.19: cyclomaltodextrin glucanotransferase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)
BS01	GLD	B	Y195 H233	Y195 H233
BS02	GLC	B	W616 W662	W616 W662
BS03	GLC	B	W616 K651 W662 N667	W616 K651 W662 N667
BS04	GLC	B	H98 Y100 W101 L197 D371	H98 Y100 W101 L197 D371
BS05	ACI	B	Y100 H140 L197 D229 E257 H327 D328	Y100 H140 L197 D229 E257 H327 D328
BS06	GAL	B	R47 Y89 S90 V92	R47 Y89 S90 V92
BS07	GAL	B	T598 A599 N627 Y633	T598 A599 N627 Y633
BS08	CA	B	D27 N29 N32 N33 G51 D53	D27 N29 N32 N33 G51 D53
BS09	CA	B	N139 I190 D199 H233	N139 I190 D199 H233

Gene Ontology

	Molecular Function
GO:0003824	catalytic activity
GO:0004556	alpha-amylase activity
GO:0016757	glycosyltransferase activity
GO:0030246	carbohydrate binding
GO:0043169	cation binding
GO:0043895	cyclomaltodextrin glucanotransferase activity
GO:0046872	metal ion binding
GO:2001070	starch binding

	Biological Process
GO:0005975	carbohydrate metabolic process

	Cellular Component
GO:0005576	extracellular region

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Molecular Function

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Biological Process

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Cellular Component

External links

PDB	RCSB:1v3m, PDBe:1v3m, PDBj:1v3m
PDBsum	1v3m
PubMed	14739329
UniProt	P05618\|CDGT_BACS0 Cyclomaltodextrin glucanotransferase (Gene Name=cgt)

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