Structure of PDB 1uws Chain B

Receptor sequence

>1uwsB (length=485) Species: 2287 (Saccharolobus solfataricus)

MYSFPNSFRFGWSQAGFQSEMGTPGSEDPNTDWYKWVHDPENMAAGLVSG
DLPENGPGYWGNYKTFHDNAQKMGLKIARLNVEWSRIFPNPLPRPFDESK
QDVTEVEINENELKRLDEYANKDALNHYREIFKDLKSRGLYFILNMYHWP
LPLWLHDPIRVRRGDFTGPSGWLSTRTVYEFARFSAYIAWKFDDLVDEYS
TMNEPNVVGGLGYVGVKSGFPPGYLSFELSRRAMYNIIQAHARAYDGIKS
VSKKPVGIIYANSSFQPLTDKDMEAVEMAENDNRWWFFDAIIRGEITREK
IVRDDLKGRLDWIGVNYYTRTVVKRTEKGYVSLGGYGHGCERNSVSLAGL
PTSDFGWEFFPEGLYDVLTKYWNRYHLYMYVTENGIADDADYQRPYYLVS
HVYQVHRAINSGADVRGYLHWSLADNYEWASGFSMRFGLLKVDYNTKRLY
WRPSALVYREIATNGAITDEIEHLNSVPPVKPLRH

3D structure

• PDB: 1uws Structural studies of the beta-glycosidase from Sulfolobus solfataricus in complex with covalently and noncovalently bound inhibitors.
• Chain: B
• Resolution: 1.95 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): R79 H150 E206 V209 N320 Y322 E387
• Catalytic site (residue number reindexed from 1): R79 H148 E204 V207 N316 Y318 E383
• Enzyme Commision number: 3.2.1.23: beta-galactosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	G2F	B	Q18 H150 N205 E206 Y322 E387 W425 E432 F441	Q18 H148 N203 E204 Y318 E383 W421 E428 F437

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds
• GO:0004565 beta-galactosidase activity
• GO:0008422 beta-glucosidase activity
• GO:0016798 hydrolase activity, acting on glycosyl bonds

Biological Process

• GO:0005975 carbohydrate metabolic process

External links

• PDB: RCSB:1uws, PDBe:1uws, PDBj:1uws
• PDBsum: 1uws
• PubMed: 15147194
• UniProt: P22498|BGAL_SACS2 Beta-galactosidase (Gene Name=lacS)