Structure of PDB 1uwk Chain B

Receptor sequence

>1uwkB (length=553) Species: 303 (Pseudomonas putida)

NKYRDVEIRAPRGNKLTAKSWLTEAPLRMLMNNLDPQVAENPKELVVYGG
IGRAARNWECYDKIVETLTRLEDDETLLVQSGKPVGVFKTHSNAPRVLIA
NSNLVPHWANWEHFNELDAKGLAMYGQMTAGSWIYIGSQGIVQGTYETFV
EAGRQHYGGSLKGKWVLTAGLGGMGGAQPLAATLAGACSLNIESQQSRID
FRLETRYVDEQATDLDDALVRIAKYTAEGKAISIALHGNAAEILPELVKR
GVRPDMVTDQTSAHDPLNGYLPAGWTWEQYRDRAQTEPAAVVKAAKQSMA
VHVQAMLDFQKQGVPTFDYGNNIRQMAKEEGVADAFDFPGFVPAYIRPLF
CRGVGPFRWAALSGEAEDIYKTDAKVKELIPDDAHLHRWLDMARERISFQ
GLPARICWVGLGLRAKLGLAFNEMVRSGELSAPVVIGRDHLDSGSVSSPN
AETEAMRDGSDAVSDWPLLNALLNTAGGATWVSLHHGGGVGMGFSQHSGM
VIVCDGTDEAAERIARVLTNDPGTGVMRHADAGYDIAIDCAKEQGLDLPM
ITG

3D structure

• PDB: 1uwk Structure and Action of Urocanase
• Chain: B
• Resolution: 1.19 Å

Enzymatic activity

• Enzyme Commision number: 4.2.1.49: urocanate hydratase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	URO	B	Y52 M132 T133 G144 M178 R362 D443 G493	Y48 M128 T129 G140 M174 R358 D439 G489
BS02	NAD	B	Y52 G53 G54 I145 G176 G177 M178 E197 S198 Q199 N243 A244 Q264 T265 S266 H268 Y274 L275 Y323 G324 N325 F345 G493	Y48 G49 G50 I141 G172 G173 M174 E193 S194 Q195 N239 A240 Q260 T261 S262 H264 Y270 L271 Y319 G320 N321 F341 G489

Gene Ontology

Molecular Function

• GO:0016153 urocanate hydratase activity
• GO:0016829 lyase activity

Biological Process

• GO:0006547 L-histidine metabolic process
• GO:0006548 L-histidine catabolic process
• GO:0019556 L-histidine catabolic process to glutamate and formamide
• GO:0019557 L-histidine catabolic process to glutamate and formate

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1uwk, PDBe:1uwk, PDBj:1uwk
• PDBsum: 1uwk
• PubMed: 15313616
• UniProt: P25080|HUTU_PSEPU Urocanate hydratase (Gene Name=hutU)