Structure of PDB 1u0d Chain B

Receptor sequence
>1u0dB (length=152) Species: 3055 (Chlamydomonas reinhardtii) [Search protein sequence]
NTKYNKEFLLYLAGFVDGDGSIIAQIKPNQSHKFKHQLSLTFQVTEKTQR
RWFLDKLVDEIGVGYVRDRGSVSDYILSEIKPLHNFLTQLQPFLKLKQKQ
ANLVLKIIEQLPSAKESPDKFLEVCTWVDQIAALNDSKTRKTTSETVRAV
LD
3D structure
PDB1u0d Isolation and characterization of new homing endonuclease specificities at individual target site positions.
ChainB
Resolution2.9 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) G319 D320
Catalytic site (residue number reindexed from 1) G18 D19
Enzyme Commision number 3.1.-.-
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B D320 S332 H333 K334 Q338 Y366 R368 R370 E380 D19 S31 H32 K33 Q37 Y65 R67 R69 E79 PDBbind-CN: Kd=2.0nM
BS02 dna B D320 S322 I324 Q326 K328 Q344 T346 E347 K348 R370 V373 N436 D437 S438 K442 D19 S21 I23 Q25 K27 Q43 T45 E46 K47 R69 V72 N135 D136 S137 K141 PDBbind-CN: Kd=2.0nM
Gene Ontology
Molecular Function
GO:0004519 endonuclease activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding
Biological Process
GO:0006314 intron homing
Cellular Component
GO:0009507 chloroplast

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1u0d, PDBe:1u0d, PDBj:1u0d
PDBsum1u0d
PubMed15313605
UniProtP05725|DNE1_CHLRE DNA endonuclease I-CreI

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