Structure of PDB 1tyt Chain B

Receptor sequence
>1tytB (length=486) Species: 5656 (Crithidia fasciculata) [Search protein sequence]
SRAYDLVVIGAGSGGLEAGWNAASLHKKRVAVIDLQKHHGPPHYAALGGT
CVNVGCVPKKLMVTGANYMDTIRESAGFGWELDRESVRPNWKALIAAKNK
AVSGINDSYEGMFADTEGLTFHQGFGALQDNHTVLVRESADPNSAVLETL
DTEYILLATGSWPQHLGIEGDDLCITSNEAFYLDEAPKRALCVGGGYISI
EFAGIFNAYKARGGQVDLAYRGDMILRGFDSELRKQLTEQLRANGINVRT
HENPAKVTKNADGTRHVVFESGAEADYDVVMLAIGRVPRSQTLQLDKAGV
EVAKNGAIKVDAYSKTNVDNIYAIGDVTDRVMLTPVAINEGAAFVDTVFA
NKPRATDHTKVACAVFSIPPMGVCGYVEEDAAKKYDQVAVYESSFTPLMH
NISGSTYKKFMVRIVTNHADGEVLGVHMLGDSSPEIIQSVAICLKMGAKI
SDFYNTIGVHPTSAEELCSMRTPAYFYQKGKRVEKI
3D structure
PDB1tyt Structure of trypanothione reductase from Crithidia fasciculata at 2.6 A resolution; enzyme-NADP interactions at 2.8 A resolution.
ChainB
Resolution2.6 Å
3D
structure
Catalytic site residues are labeled in the structure
[Spin on]
[Spin off]
[Reset orientation]

[High quality]
[Low quality]

[White background]
[Black background]

[Download]
[Download structure with residue number starting from 1]
Enzymatic activity
Catalytic site (original residue number in PDB) S14 L48 C52 C57 K60 R85 E86 Y198 E202 I339 G459 H461 E466 E485 K486
Catalytic site (residue number reindexed from 1) S13 L47 C51 C56 K59 R84 E85 Y197 E201 I338 G458 H460 E465 E484 K485
Enzyme Commision number 1.8.1.12: trypanothione-disulfide reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 FAD B G11 G13 S14 G15 D35 L36 A46 G50 T51 C52 V55 G56 C57 K60 F126 G127 T160 G161 S178 Y198 R287 R290 G326 D327 M333 L334 T335 G10 G12 S13 G14 D34 L35 A45 G49 T50 C51 V54 G55 C56 K59 F125 G126 T159 G160 S177 Y197 R286 R289 G325 D326 M332 L333 T334
Gene Ontology
Molecular Function
GO:0004362 glutathione-disulfide reductase (NADPH) activity
GO:0015036 disulfide oxidoreductase activity
GO:0015042 trypanothione-disulfide reductase (NADPH) activity
GO:0016491 oxidoreductase activity
GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
GO:0050660 flavin adenine dinucleotide binding
Biological Process
GO:0006749 glutathione metabolic process
GO:0034599 cellular response to oxidative stress
GO:0045454 cell redox homeostasis
GO:0098869 cellular oxidant detoxification
Cellular Component
GO:0005737 cytoplasm
GO:0005739 mitochondrion
GO:0005829 cytosol

View graph for
Molecular Function
View graph for
Biological Process
View graph for
Cellular Component
External links
PDB RCSB:1tyt, PDBe:1tyt, PDBj:1tyt
PDBsum1tyt
PubMed15299452
UniProtP39040|TYTR_CRIFA Trypanothione reductase (Gene Name=TPR)

[Back to BioLiP]