Structure of PDB 1t90 Chain B

Receptor sequence
>1t90B (length=484) Species: 1423 (Bacillus subtilis) [Search protein sequence]
EIRKLKNYINGEWVESKTDQYEDVVNPATKEVLCQVPISTKEDIDYAAQT
AAEAFKTWSKVAVPRRARILFNFQQLLSQHKEELAHLITIENGKNTKEAL
GEVGRGIENVEFAAGAPSLMMGDSLASIATDVEAANYRYPIGVVGGIAPF
NFPMMVPCWMFPMAIALGNTFILKPSERTPLLTEKLVELFEKAGLPKGVF
NVVYGAHDVVNGILEHPEIKAISFVGSKPVGEYVYKKGSENLKRVQSLTG
AKNHTIVLNDANLEDTVTNIVGAAFGSAGERCMACAVVTVEEGIADEFMA
KLQEKVADIKIGNGLDDGVFLGPVIREDNKKRTLSYIEKGLEEGARLVCD
GRENVSDDGYFVGPTIFDNVTTEMTIWKDEIFAPVLSVIRVKNLKEAIEI
ANKSEFANGACLFTSNSNAIRYFRENIDAGMLGINLGVPAPMAFFPFSGW
KSSFFGTLHANGKDSVDFYTRKKVVTARYPAPDF
3D structure
PDB1t90 Expression, purification, crystallization and preliminary X-ray diffraction data of Methylmalonate-Semialdehyde Dehydrogenase from Bacillus subtilis
ChainB
Resolution2.5 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) N153 K176 L250 C284 E382 A462
Catalytic site (residue number reindexed from 1) N151 K174 L248 C282 E380 A460
Enzyme Commision number 1.2.1.27: methylmalonate-semialdehyde dehydrogenase (CoA acylating).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 NAD B I149 A150 P151 F152 N153 W161 K176 R180 H209 V212 F226 V227 G228 S229 V232 L250 T251 C284 E382 F384 I147 A148 P149 F150 N151 W159 K174 R178 H207 V210 F224 V225 G226 S227 V230 L248 T249 C282 E380 F382
Gene Ontology
Molecular Function
GO:0004491 methylmalonate-semialdehyde dehydrogenase (acylating, NAD) activity
GO:0016491 oxidoreductase activity
GO:0016620 oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor
GO:0018478 malonate-semialdehyde dehydrogenase (acetylating) activity
Biological Process
GO:0006210 thymine catabolic process
GO:0006574 valine catabolic process
GO:0019310 inositol catabolic process

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Molecular Function
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Biological Process
External links
PDB RCSB:1t90, PDBe:1t90, PDBj:1t90
PDBsum1t90
PubMed
UniProtP42412|IOLA_BACSU Malonate-semialdehyde dehydrogenase (Gene Name=iolA)

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