Structure of PDB 1t3n Chain B

Receptor sequence

>1t3nB (length=388) Species: 9606 (Homo sapiens)

SRVIVHVDLDCFYAQVEMISNPELKDKPLGVQQKYLVVTCNYEARKLGVK
KLMNVRDAKEKCPQLVLVNGEDLTRYREMSYKVTELLEEFSPVVERLGFD
ENFVDLTEMVEKRLQQLQSDELSAVTVSGHVYNNQSINLLDVLHIRLLVG
SQIAAEMREAMYNQLGLTGCAGVASNKLLAKLVSGVFKPNQQTVLLPESC
QHLIHSLNHIKEIPGIGYKTAKCLEALGINSVRDLQTFSPKILEKELGIS
VAQRIQKLSFGEDNSPVILSGPPQSFSEEDSFKKCSSEVEAKNKIEELLA
SLLNRVCQDGRKPHTVRLIIRRYSSEKHYGRESRQCPIPSHVIQKLGTGN
YDVMTPMVDILMKLFRNMVNVKMPFHLTLLSVCFCNLK

3D structure

• PDB: 1t3n Replication by human DNA polymerase-iota occurs by Hoogsteen base-pairing.
• Chain: B
• Resolution: 2.3 Å

Enzymatic activity

• Enzyme Commision number: 2.7.7.7: DNA-directed DNA polymerase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	dna	B	Q479 K480 L482 E517 L519 R523 Q720 S721 S723 E724 E725 S727 L825	Q33 K34 L36 E71 L73 R77 Q274 S275 S277 E278 E279 S281 L379
BS02	dna	B	L543 E547 K627 G663 K665 R763 Y775 G776 R777 E778 S779 R780 Q781 P783	L97 E101 K181 G217 K219 R317 Y329 G330 R331 E332 S333 R334 Q335 P337
BS03	MG	B	D454 L455 D546	D8 L9 D100
BS04	TTP	B	L455 D456 C457 F458 Y459 V484 T485 Y488 R491 L498 D546 K634	L9 D10 C11 F12 Y13 V38 T39 Y42 R45 L52 D100 K188

Gene Ontology

Molecular Function

• GO:0003684 damaged DNA binding
• GO:0003887 DNA-directed DNA polymerase activity

Biological Process

• GO:0006281 DNA repair

External links

• PDB: RCSB:1t3n, PDBe:1t3n, PDBj:1t3n
• PDBsum: 1t3n
• PubMed: 15254543
• UniProt: Q9UNA4|POLI_HUMAN DNA polymerase iota (Gene Name=POLI)