Structure of PDB 1sx8 Chain B

Receptor sequence
>1sx8B (length=235) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIATTYTNKIKF
TLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVSLKTYNINELNEIP
KPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVEGKGIFDSED
EFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1sx8 DNA Cleavage by EcoRV Endonuclease: Two Metal Ions in Three Metal Ion Binding Sites
ChainB
Resolution2.15 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B Q69 N70 H71 S183 G184 N185 T222 S223 Q68 N69 H70 S173 G174 N175 T212 S213
BS02 dna B T37 N70 A92 T93 T94 T106 G109 T111 K119 N120 G182 S183 T186 N188 T36 N69 A91 T92 T93 T101 G104 T106 K114 N115 G172 S173 T176 N178
BS03 MN B D74 D90 D73 D89
BS04 MN B E45 D74 E44 D73
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1sx8, PDBe:1sx8, PDBj:1sx8
PDBsum1sx8
PubMed15170321
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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