Structure of PDB 1shn Chain B

Receptor sequence

>1shnB (length=476) Species: 6703 (Pandalus borealis)

EEDKAYWNKDAQDALDKQLGIKLREKQAKNVIFFLGDGMSLSTVTAARIY
KGGLTGKFEREKISWEEFDFAALSKTYNTDKQVTDSAASATAYLTGVKTN
QGVIGLDANTVRTNCSYQLDESLFTYSIAHWFQEAGRSTGVVTSTRVTHA
TPAGTYAHVADRDWENDSDVVHDREDPEICDDIAEQLVFREPGKNFKVIM
GGGRRGFFPEEALDIEDGIPGEREDGKHLITDWLDDKASQGATASYVWNR
DDLLAVDIRNTDYLMGLFSYTHLDTVLTRDAEMDPTLPEMTKVAIEMLTK
DENGFFLLVEGGRIDHMHHANQIRQSLAETLDMEEAVSMALSMTDPEETI
ILVTADHGHTLTITGYADRNTDILDFAGISDLDDRRYTILDYGSGPGYHI
TEDGKRYEPTEEDLKDINFRYASAAPKHSVTHDGTDVGIWVNGPFAHLFT
GVYEENYIPHALAYAACVGTGRTFCD

3D structure

• PDB: 1shn Ligand-binding and metal-exchange crystallographic studies on shrimp alkaline phosphatase.
• Chain: B
• Resolution: 2.15 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D37 S86 H149 T151 R162 E310 D315 H316 H319 D356 H357 H432
• Catalytic site (residue number reindexed from 1): D37 S86 H149 T151 R162 E310 D315 H316 H319 D356 H357 H432
• Enzyme Commision number: 3.1.3.1: alkaline phosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	D315 H319 H432	D315 H319 H432
BS02	ZN	B	D37 S86 D356 H357	D37 S86 D356 H357
BS03	ZN	B	D37 H149 T151 E310	D37 H149 T151 E310
BS04	PO4	B	S86 R162 D315 H319 H357 H432	S86 R162 D315 H319 H357 H432

Gene Ontology

Molecular Function

• GO:0004035 alkaline phosphatase activity
• GO:0016787 hydrolase activity
• GO:0016791 phosphatase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0016311 dephosphorylation

Cellular Component

• GO:0098552 side of membrane

External links

• PDB: RCSB:1shn, PDBe:1shn, PDBj:1shn
• PDBsum: 1shn
• PubMed: 15333925
• UniProt: Q9BHT8