Structure of PDB 1sez Chain B

Receptor sequence

>1sezB (length=465) Species: 4097 (Nicotiana tabacum)

AKRVAVIGAGVSGLAAAYKLKIHGLNVTVFEAEGKAGGKLRSVSQDGLIW
DEGANTMTESEGDVTFLIDSLGLREKQQFPLSQNKRYIARNGTPVLLPSN
PIDLIKSNFLSTGSKLQMLLEPILWSHESVSGFFQRHFGKEVVDYLIDPF
VAGTCGGDPDSLSMHHSFPELWNLEKRFGSVILGAIRSKLSKTSANKKRQ
RGSFSFLGGMQTLTDAICKDLREDELRLNSRVLELSCSCTEDSAIDSWSI
ISASPHKRQSEEESFDAVIMTAPLCDVKSMKIAKRGNPFLLNFIPEVDYV
PLSVVITTFKRENVKYPLEGFGVLVPSKEQQHGLKTLGTLFSSMMFPDRA
PNNVYLYTTFVGGSRNRELAKASRTELKEIVTSDLKQLLGAEGEPTYVNH
LYWSKAFPLYGHNYDSVLDAIDKMEKNLPGLFYAGNHRGGLSVGKALSSG
CNAADLVISYLESVS

3D structure

• PDB: 1sez Crystal structure of protoporphyrinogen IX oxidase: a key enzyme in haem and chlorophyll biosynthesis.
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Enzyme Commision number: 1.3.3.4: protoporphyrinogen oxidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	G20 G22 S24 F42 E43 A44 G50 K51 G65 A66 N67 T68 R263 V264 T303 A304 D308 A438 F439 N468 S474 V475	G8 G10 S12 F30 E31 A32 G38 K39 G53 A54 N55 T56 R231 V232 T271 A272 D276 A406 F407 N436 S442 V443
BS02	OMN	B	R98 A174 G175 G178 L334 G354 V355 L356 L369 G370 T371 L372 F392	R86 A152 G153 G156 L302 G322 V323 L324 L337 G338 T339 L340 F360
BS03	TON	B	F172 F190	F150 F168

Gene Ontology

Molecular Function

• GO:0004729 oxygen-dependent protoporphyrinogen oxidase activity
• GO:0016491 oxidoreductase activity

Biological Process

• GO:0006779 porphyrin-containing compound biosynthetic process
• GO:0006782 protoporphyrinogen IX biosynthetic process
• GO:0006783 heme biosynthetic process

Cellular Component

• GO:0005739 mitochondrion
• GO:0005743 mitochondrial inner membrane

External links

• PDB: RCSB:1sez, PDBe:1sez, PDBj:1sez
• PDBsum: 1sez
• PubMed: 15057273
• UniProt: O24164|PPOM_TOBAC Protoporphyrinogen oxidase, mitochondrial (Gene Name=PPXII)