Structure of PDB 1s16 Chain B

Receptor sequence
>1s16B (length=380) Species: 562 (Escherichia coli) [Search protein sequence]
TYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSVDEALAGHAK
RVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSNK
NYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVV
GTCGKRNTGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFK
DEINNTEQRWCYQDGLNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWAL
LWLPEGGELLTESYVNLIPTMQGGTHVNGLRQGLLDAMREFCEYRNILPR
GVKLSAEDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFVSGVVKDA
FILWLNQNVQAAELLAEMAISSAQRRMRAA
3D structure
PDB1s16 Crystal structures of Escherichia coli topoisomerase IV ParE subunit (24 and 43 kilodaltons): a single residue dictates differences in novobiocin potency against topoisomerase IV and DNA gyrase.
ChainB
Resolution2.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 5.6.2.2: DNA topoisomerase (ATP-hydrolyzing).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B I2090 R2093 A2096 G2113 S2117 I87 R90 A93 G110 S114
BS02 ANP B N2042 E2046 M2074 I2090 G2097 G2098 K2099 Y2105 G2110 L2111 H2112 G2113 V2114 G2115 I2116 K2334 N39 E43 M71 I87 G94 G95 K96 Y102 G107 L108 H109 G110 V111 G112 I113 K331
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
GO:0005524 ATP binding
Biological Process
GO:0006265 DNA topological change
Cellular Component
GO:0005694 chromosome

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1s16, PDBe:1s16, PDBj:1s16
PDBsum1s16
PubMed15105144
UniProtP20083|PARE_ECOLI DNA topoisomerase 4 subunit B (Gene Name=parE)

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