Structure of PDB 1rvc Chain B

Receptor sequence
>1rvcB (length=244) Species: 562 (Escherichia coli) [Search protein sequence]
SLRSDLINALYDENQKYDVCGIISAEGKIYPLGSDTKVLSTIFELFSRPI
INKIAEKHGYIVEEPKQQNHYPDFTLYKPSEPNKKIAIDIKTTYTNKENE
KIKFTLGGYTSFIRNNTKNIVYPFDQYIAHWIIGYVYTRVATRKSSLKTY
NINELNEIPKPYKGVKVFLQDKWVIAGDLAGSGNTTNIGSIHAHYKDFVE
GKGIFDSEDEFLDYWRNYERTSQLRNDKYNNISEYRNWIYRGRK
3D structure
PDB1rvc Mg2+ binding to the active site of EcoRV endonuclease: a crystallographic study of complexes with substrate and product DNA at 2 A resolution.
ChainB
Resolution2.1 Å
3D
structure
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Enzymatic activity
Enzyme Commision number 3.1.21.4: type II site-specific deoxyribonuclease.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 dna B S183 G184 N185 S223 S182 G183 N184 S222
BS02 dna B K38 Q68 Q69 N70 K37 Q67 Q68 N69
BS03 dna B N70 G109 T111 S112 K119 N120 T186 N69 G108 T110 S111 K118 N119 T185
BS04 dna B T37 K92 T93 T94 Y95 S183 T186 N188 T36 K91 T92 T93 Y94 S182 T185 N187
BS05 MG B E45 D74 E44 D73
Gene Ontology
Molecular Function
GO:0003677 DNA binding
GO:0004519 endonuclease activity
GO:0009036 type II site-specific deoxyribonuclease activity
GO:0046872 metal ion binding
Biological Process
GO:0009307 DNA restriction-modification system

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Molecular Function
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Biological Process
External links
PDB RCSB:1rvc, PDBe:1rvc, PDBj:1rvc
PDBsum1rvc
PubMed7819264
UniProtP04390|T2E5_ECOLX Type II restriction enzyme EcoRV (Gene Name=ecoRVR)

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