Structure of PDB 1r30 Chain B

Receptor sequence

>1r30B (length=313) Species: 562 (Escherichia coli)

HRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVSTLLSIKTGA
CPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNH
NLDTSPEFYGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDR
AGLLLQLANLPTPPESVPINMLVKVKGTPLADNDDVDAFDFIRTIAVARI
MMPTSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDL
QLFRKLGLNPQQT

3D structure

• PDB: 1r30 Crystal structure of biotin synthase, an S-adenosylmethionine-dependent radical enzyme.
• Chain: B
• Resolution: 3.4 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C53 C57 C60 C97 C128 C188 R260
• Catalytic site (residue number reindexed from 1): C51 C55 C58 C95 C126 C186 R258
• Enzyme Commision number: 2.8.1.6: biotin synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	SAM	B	Y59 G132 N153 D155 R173 I192 L224 V225 L291	Y57 G130 N151 D153 R171 I190 L222 V223 L289
BS02	SF4	B	C53 E55 C57 C60	C51 E53 C55 C58
BS03	FES	B	C97 C128 C188 R260	C95 C126 C186 R258
BS04	DTB	B	N151 N222 A263 F285 L291 T292 T293	N149 N220 A261 F283 L289 T290 T291

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004076 biotin synthase activity
• GO:0005506 iron ion binding
• GO:0005515 protein binding
• GO:0016740 transferase activity
• GO:0042803 protein homodimerization activity
• GO:0046872 metal ion binding
• GO:0051536 iron-sulfur cluster binding
• GO:0051537 2 iron, 2 sulfur cluster binding
• GO:0051539 4 iron, 4 sulfur cluster binding

Biological Process

• GO:0009102 biotin biosynthetic process
• GO:0042364 water-soluble vitamin biosynthetic process
• GO:0044272 sulfur compound biosynthetic process

External links

• PDB: RCSB:1r30, PDBe:1r30, PDBj:1r30
• PDBsum: 1r30
• PubMed: 14704425
• UniProt: P12996|BIOB_ECOLI Biotin synthase (Gene Name=bioB)