Structure of PDB 1qw8 Chain B

Receptor sequence

>1qw8B (length=497) Species: 1422 (Geobacillus stearothermophilus)

KATMIIEKDFKIAEIDKRIYGSFIEHLGRAVYGGIYEPGHPQADENGFRQ
DVIELVKELQVPIIRYPGGNFVSGYNWEDGVGPKEQRPRRLDLAWKSVET
NEIGLNEFMDWAKMVGAEVNMAVNLGTRGIDAARNLVEYCNHPSGSYYSD
LRIAHGYKEPHKIKTWCLGNAMDGPWQIGHKTAVEYGRIACEAAKVMKWV
DPTIELVVCGSSNRNMPTFAEWEATVLDHTYDHVDYISLHQYYGNRDNDT
ANYLALSLEMDDFIRSVVAIADYVKAKKRSKKTIHLSFDEWNVWYHSNEA
DKLIEPWTVAPPLLEDIYNFEDALLVGCMLITLMKHADRVKIACLAQLVN
VIAPIMTEKNGPAWKQTIYYPFMHASVYGRGVALHPVISSPKYDSKDFTD
VPYLESIAVYNEEKEEVTIFAVNRDMEDALLLECDVRSFEDYRVIEHIVL
EHDNVKQTNSAQSSPVVPHRNGDAQLSDRKVSATLPKLSWNVIRLGK

3D structure

• PDB: 1qw8 Crystal structure and snapshots along the reaction pathway of a family 51 alpha-L-arabinofuranosidase
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): A175 E294
• Catalytic site (residue number reindexed from 1): A171 E290
• Enzyme Commision number: 3.2.1.55: non-reducing end alpha-L-arabinofuranosidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	AHR	B	E29 G73 N74 N174 Y246 E294 Q351	E25 G69 N70 N170 Y242 E290 Q347

Gene Ontology

Molecular Function

• GO:0016798 hydrolase activity, acting on glycosyl bonds
• GO:0046556 alpha-L-arabinofuranosidase activity

Biological Process

• GO:0000272 polysaccharide catabolic process
• GO:0031222 arabinan catabolic process
• GO:0046373 L-arabinose metabolic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1qw8, PDBe:1qw8, PDBj:1qw8
• PDBsum: 1qw8
• PubMed: 14517232
• UniProt: Q9XBQ3|IABF_GEOSE Intracellular exo-alpha-(1->5)-L-arabinofuranosidase (Gene Name=abfA)