Structure of PDB 1pjs Chain B

Receptor sequence
>1pjsB (length=454) [Search protein sequence]
MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLEAGARLTVNALTFIPQFT
VWANEGMLTLVEGPFDETLLDSCWLAIAATDDDTVNQRVSDAAESRRIFC
NVVDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPQH
LGQVARYAGQLRARVKKQFATMGERRRFWEKFFVNDRLAQSLANADEKAV
NATTERLFSEPLDHRGEVVLVGAGPGDAGLLTLKGLQQIQQADIVVYDRL
VSDDIMNLVRRDADRVFVGKRAGYHCVPQEEINQILLREAQKGKRVVRLK
GGDPFIFGRGGEELETLCHAGIPFSVVPGITAASGCSAYSGIPLTHRDYA
QSVRLVTGHGGELDWENLAAEKQTLVFYMGLNQAATIQEKLIAFGMQADM
PVALVENGTSVKQRVVHGVLTQLGELAQQVESPALIIVGRVVALRDKLNW
FSNH
3D structure
PDB1pjs CysG structure reveals tetrapyrrole-binding features and novel regulation of siroheme biosynthesis.
ChainB
Resolution2.4 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) D248 K270 M382
Catalytic site (residue number reindexed from 1) D248 K270 M379
Enzyme Commision number 1.3.1.76: precorrin-2 dehydrogenase.
2.1.1.107: uroporphyrinogen-III C-methyltransferase.
4.99.1.4: sirohydrochlorin ferrochelatase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 SAH B G301 D303 I306 F307 T331 A332 C336 Y381 M382 N410 G411 P436 A437 L438 G301 D303 I306 F307 T331 A332 C336 Y378 M379 N407 G408 P433 A434 L435
BS02 NAD B G20 G21 D22 V23 L43 T44 T80 D81 D104 G20 G21 D22 V23 L43 T44 T80 D81 D104
Gene Ontology
Molecular Function
GO:0004325 ferrochelatase activity
GO:0004851 uroporphyrin-III C-methyltransferase activity
GO:0008168 methyltransferase activity
GO:0016491 oxidoreductase activity
GO:0016829 lyase activity
GO:0043115 precorrin-2 dehydrogenase activity
GO:0051266 sirohydrochlorin ferrochelatase activity
GO:0051287 NAD binding
Biological Process
GO:0006779 porphyrin-containing compound biosynthetic process
GO:0009236 cobalamin biosynthetic process
GO:0019354 siroheme biosynthetic process
GO:0032259 methylation

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Molecular Function
View graph for
Biological Process
External links
PDB RCSB:1pjs, PDBe:1pjs, PDBj:1pjs
PDBsum1pjs
PubMed14595395
UniProtP25924|CYSG_SALTY Siroheme synthase (Gene Name=cysG)

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