Structure of PDB 1pj4 Chain B

Receptor sequence
>1pj4B (length=552) Species: 9606 (Homo sapiens) [Search protein sequence]
KEKGKPLMLNPRTNKGMAFTLQERQMLGLQGLLPPKIETQDIQALRFHRN
LKKMTSPLEKYIYIMGIQERNEKLFYRILQDDIESLMPIVYTPTVGLACS
QYGHIFRRPKGLFISISDRGHVRSIVDNWPENHVKAVVVTDGERILGLGD
LGVYGMGIPVGKLCLYTACAGIRPDRCLPVCIDVGTDNIALLKDPFYMGL
YQKRDRTQQYDDLIDEFMKAITDRYGRNTLIQFEDFGNHNAFRFLRKYRE
KYCTFNDDIQGTAAVALAGLLAAQKVISKPISEHKILFLGAGEAALGIAN
LIVMSMVENGLSEQEAQKKIWMFDKYGLLVKGRKAKIDSYQEPFTHSAPE
SIPDTFEDAVNILKPSTIIGVAGAGRLFTPDVIRAMASINERPVIFALSN
PTAQAECTAEEAYTLTEGRCLFASGSPFGPVKLTDGRVFTPGQGNNVYIF
PGVALAVILCNTRHISDSVFLEAAKALTSQLTDEELAQGRLYPPLANIQE
VSINIAIKVTEYLYANKMAFRYPEPEDKAKYVKERTWRSEYDSLLPDVYE
WP
3D structure
PDB1pj4 Crystal structures of substrate complexes of malic enzyme and insights into the catalytic mechanism.
ChainB
Resolution2.3 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) Y1112 R1165 K1183 E1255 D1256 D1278 D1279 N1421
Catalytic site (residue number reindexed from 1) Y91 R144 K162 E234 D235 D257 D258 N400
Enzyme Commision number 1.1.1.38: malate dehydrogenase (oxaloacetate-decarboxylating).
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MN B E1255 D1256 D1279 E234 D235 D258
BS02 ATP B R1165 N1259 G1311 A1312 G1313 E1314 D1345 K1346 V1392 A1393 G1394 R144 N238 G290 A291 G292 E293 D324 K325 V371 A372 G373
BS03 ATP B K1156 R1194 R1197 I1479 L1480 R1542 R1556 K135 R173 R176 I458 L459 R521 R535
BS04 FUM B R1067 R1091 R46 R70
Gene Ontology
Molecular Function
GO:0004470 malic enzyme activity
GO:0004471 malate dehydrogenase (decarboxylating) (NAD+) activity
GO:0004473 malate dehydrogenase (decarboxylating) (NADP+) activity
GO:0008948 oxaloacetate decarboxylase activity
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0046872 metal ion binding
GO:0051287 NAD binding
Biological Process
GO:0006090 pyruvate metabolic process
GO:0006108 malate metabolic process
GO:1902031 regulation of NADP metabolic process
Cellular Component
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0043231 intracellular membrane-bounded organelle

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Cellular Component
External links
PDB RCSB:1pj4, PDBe:1pj4, PDBj:1pj4
PDBsum1pj4
PubMed12962632
UniProtP23368|MAOM_HUMAN NAD-dependent malic enzyme, mitochondrial (Gene Name=ME2)

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