Structure of PDB 1p6l Chain B

Receptor sequence

>1p6lB (length=403) Species: 9913 (Bos taurus)

KFPRVKNWELGSITYDTLCAQSQQDGPCTPRRCLGSLVLPRPAEQLLSQA
RDFINQYYSSIKRSGSQAHEERLQEVEAEVASTGTYHLRESELVFGAKQA
WRNAPRCVGRIQWGKLQVFDARDCSSAQEMFTYICNHIKYATNRGNLRSA
ITVFPQRAPGRGDFRIWNSQLVRYAGYRQQDGSVRGDPANVEITELCIQH
GWTPGNGRFDVLPLLLQAPDEAPELFVLPPELVLEVPLEHPTLEWFAALG
LRWYALPAVSNMLLEIGGLEFSAAPFSGWYMSTEIGTRNLCDPHRYNILE
DVAVCMDLDTRTTSSLWKDKAAVEINLAVLHSFQLAKVTIVDHHAATVSF
MKHLDNEQKARGGCPADWAWIVPPISGSLTPVFHQEMVNYILSPAFRYQP
DPW

3D structure

• PDB: 1p6l Structural basis for dipeptide amide isoform-selective inhibition of neuronal nitric oxide synthase.
• Chain: B
• Resolution: 2.35 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C186 R189 W358 E363
• Catalytic site (residue number reindexed from 1): C107 R110 W279 E284
• Enzyme Commision number: 1.14.13.39: nitric-oxide synthase (NADPH).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	H4B	B	F462 H463 Q464 E465	F383 H384 Q385 E386
BS02	ZN	B	C96 C101	C28 C33
BS03	HEM	B	W180 R185 C186 M341 F355 W358 E363 W449 Y477	W101 R106 C107 M262 F276 W279 E284 W370 Y398
BS04	H4B	B	S104 R367 A448 W449	S36 R288 A369 W370
BS05	DP1	B	Q249 N340 S356 G357 W358 E363	Q170 N261 S277 G278 W279 E284	MOAD: Ki=107uM

Gene Ontology

Molecular Function

• GO:0004517 nitric-oxide synthase activity
• GO:0020037 heme binding

Biological Process

• GO:0006809 nitric oxide biosynthetic process

External links

• PDB: RCSB:1p6l, PDBe:1p6l, PDBj:1p6l
• PDBsum: 1p6l
• PubMed: 14718923
• UniProt: P29473|NOS3_BOVIN Nitric oxide synthase 3 (Gene Name=NOS3)