Structure of PDB 1p1j Chain B

Receptor sequence

>1p1jB (length=516) Species: 4932 (Saccharomyces cerevisiae)

TSVKVVTDKCTYKDNELLTKYSYENAVVTKTASGRFDVTPTVQDYVFKLD
LKKPEKLGIMLIGLGGNNGSTLVASVLANKHNVEFQTKEGVKQPNYFGSM
TQCSTLKLGIDAEGNDVYAPFNSLLPMVSPNDFVVSGWDINNADLYEAMQ
RSQVLEYDLQQRLKAKMSLVKPLPSIYYPDFIAANQDERANNCINLDEKG
NVTTRGKWTHLQRIRRDIQNFKEENALDKVIVLWTANTERYVEVSPGVND
TMENLLQSIKNDHEEIAPSTIFAAASILEGVPYINGSPQNTFVPGLVQLA
EHEGTFIAGDDLKSGQTKLKSVLAQFLVDAGIKPVSIASYNHLGNNDGYN
LSAPKQFRSKEISKSSVIDDIIASNDILYNDKLGKKVDHCIVIKYMKPVG
DSKVAMDEYYSELMLGGHNRISIHNVCEDSLLATPLIIDLLVMTEFCTRV
SYKKVKFENFYPVLTFLSYWLKAPLTRPGFHPVNGLNKQRTALENFLRLL
IGLPSQNELRFEERLL

3D structure

• PDB: 1p1j Structures of NAD(+)- and NADH-bound 1-l-myo-inositol 1-phosphate synthase.
• Chain: B
• Resolution: 1.7 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): D320 K369 K412 K489
• Catalytic site (residue number reindexed from 1): D311 K360 K403 K472
• Enzyme Commision number: 5.5.1.4: inositol-3-phosphate synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PO4	B	S323 G324 Q325 T326 K373 K412 K489	S314 G315 Q316 T317 K364 K403 K472
BS02	NAI	B	G72 G75 N76 N77 D148 I149 S184 R198 T244 A245 N246 T247 G295 D320 L321 S323 N355 D356 K369 D438 A442	G63 G66 N67 N68 D139 I140 S175 R189 T235 A236 N237 T238 G286 D311 L312 S314 N346 D347 K360 D429 A433
BS03	GOL	B	D356 K369 K373 K412 D438	D347 K360 K364 K403 D429

Gene Ontology

Molecular Function

• GO:0004512 inositol-3-phosphate synthase activity
• GO:0016853 isomerase activity

Biological Process

• GO:0006021 inositol biosynthetic process
• GO:0008654 phospholipid biosynthetic process

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1p1j, PDBe:1p1j, PDBj:1p1j
• PDBsum: 1p1j
• PubMed: 12832758
• UniProt: P11986|INO1_YEAST Inositol-3-phosphate synthase (Gene Name=INO1)