Structure of PDB 1p0f Chain B

Receptor sequence

>1p0fB (length=372) Species: 8403 (Pelophylax perezi)

CTAGKDITCKAAVAWEPHKPLSLETITVAPPKAHEVRIKILASGICGSDS
SVLKEIIPSKFPVILGHEAVGVVESIGAGVTCVKPGDKVIPLFVPQCGSC
RACKSSNSNFCEKNDMGAKTGLMADMTSRFTCRGKPIYNLMGTSTFTEYT
VVADIAVAKIDPKAPLESCLIGCGFATGYGAAVNTAKVTPGSTCAVFGLG
GVGFSAIVGCKAAGASRIIGVGTHKDKFPKAIELGATECLNPKDYDKPIY
EVICEKTNGGVDYAVECAGRIETMMNALQSTYCGSGVTVVLGLASPNERL
PLDPLLLLTGRSLKGSVFGGFKGEEVSRLVDDYMKKKINVNFLVSTKLTL
DQINKAFELLSSGQGVRSIMIY

3D structure

• PDB: 1p0f Crystal structure of the vertebrate NADP(H)-dependent alcohol dehydrogenase (ADH8)
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C2046 G2047 S2048 S2051 H2067 E2068 C2097 C2100 C2103 C2111 D2115 C2173 T2177 R2367
• Catalytic site (residue number reindexed from 1): C46 G47 S48 S51 H67 E68 C97 C100 C103 C111 D115 C173 T177 R367
• Enzyme Commision number: 1.1.1.2: alcohol dehydrogenase (NADP(+)).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	ZN	B	C2046 H2067 C2173	C46 H67 C173
BS02	ZN	B	C2097 C2100 C2103 C2111	C97 C100 C103 C111
BS03	NAP	B	C2046 G2047 S2048 C2173 T2177 L2199 G2200 G2201 V2202 G2222 T2223 H2224 K2227 C2267 A2268 R2270 L2291 V2317 R2367	C46 G47 S48 C173 T177 L199 G200 G201 V202 G222 T223 H224 K227 C267 A268 R270 L291 V317 R367

Gene Ontology

Molecular Function

• GO:0004745 all-trans-retinol dehydrogenase (NAD+) activity
• GO:0008106 alcohol dehydrogenase (NADP+) activity
• GO:0008270 zinc ion binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0042572 retinol metabolic process
• GO:0042573 retinoic acid metabolic process

Cellular Component

• GO:0005829 cytosol

External links

• PDB: RCSB:1p0f, PDBe:1p0f, PDBj:1p0f
• PDBsum: 1p0f
• PubMed: 12818203
• UniProt: O57380|ADH8_PELPE NADP-dependent alcohol dehydrogenase (Gene Name=ADH8)