Structure of PDB 1ocb Chain B

Receptor sequence

>1ocbB (length=361) Species: 34413 (Humicola insolens)

NGNPFEGVQLWANNYYRSEVHTLAIPQITDPALRAAASAVAEVPSFQWLD
RNVTVDTLLVQTLSEIREANQAGANPQYAAQIVVYDLPDRDCAAAASNGE
WAIANNGVNNYKAYINRIREILISFSDVRTILVIEPDSLANMVTNMNVPK
CSGAASTYRELTIYALKQLDLPHVAMYMDAGHAGWLGWPANIQPAAELFA
KIYEDAGKPRAVRGLATNVANYNAWSVSSPPPYTSPNPNYDEKHYIEAFR
PLLEARGFPAQFIVDQGRSGKQPTGQKEWGHWCNAIGTGFGMRPTANTGH
QYVDAFVWVKPGGECDGTSDTTAARYDYHCGLEDALKPAPEAGQWFNEYF
IQLLRNANPPF

3D structure

• PDB: 1ocb Structural Basis for Ligand Binding and Processivity in Cellobiohydrolase Cel6A from Humicola Insolens
• Chain: B
• Resolution: 1.75 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): Y174 R179 D180 S186 D226 D405
• Catalytic site (residue number reindexed from 1): Y85 R90 D91 S97 D137 D316
• Enzyme Commision number: 3.2.1.91: cellulose 1,4-beta-cellobiosidase (non-reducing end).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	G273 W274	G184 W185
BS02	BGC	B	N234 H271 W274	N145 H182 W185
BS03	GDA	B	D180 D226 N310 W371	D91 D137 N221 W282
BS04	GTM	B	W137 D139 S186 K399 P400 E403	W48 D50 S97 K310 P311 E314
BS05	BGC	B	D139 R140 E403 G432	D50 R51 E314 G343
BS06	BGC	B	R140 Q433	R51 Q344
BS07	FLG	B	Y104 S107 E108 L147 Q150	Y15 S18 E19 L58 Q61

Gene Ontology

Molecular Function

• GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds

Biological Process

• GO:0005975 carbohydrate metabolic process
• GO:0030245 cellulose catabolic process

External links

• PDB: RCSB:1ocb, PDBe:1ocb, PDBj:1ocb
• PDBsum: 1ocb
• PubMed: 12842048
• UniProt: Q9C1S9|GUX6_HUMIN Exoglucanase-6A (Gene Name=cel6A)