Structure of PDB 1nv7 Chain B

Receptor sequence

>1nv7B (length=327) Species: 9823 (Sus scrofa) [Search protein sequence]

NIVTLTRFVMEEGRKARGTGEMTQLLNSLCTAVKAISTAVRKAGIAHLYG
IAGSTNVTGDQVKKLDVLSNDLVINVLKSSFATCVLVSEEDKNAIIVEPE
KRGKYVVCFDPLDGSSNIDCLVSIGTIFGIYRKNSTDEPSEKDALQPGRN
LVAAGYALYGSATMLVLAMVNGVNCFMLDPAIGEFILVDRDVKIKKKGSI
YSINEGYAKEFDPAITEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVY
GGIFMYPANKKSPKGKLRLLYECNPMAYVMEKAGGLATTGKEAVLDIVPT
DIHQRAPIILGSPEDVTELLEIYQKHA

3D structure

PDB

1nv7 Interaction of Tl+ with product complexes of fructose-1,6-bisphosphatase

Chain

Resolution

2.15 Å

3D
structure

Catalytic site residues are labeled in the structure
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Enzymatic activity

Catalytic site (original residue number in PDB)	D2068 D2074 E2097 E2098 D2118 L2120 D2121 E2280
Catalytic site (residue number reindexed from 1)	D60 D66 E89 E90 D110 L112 D113 E272
Enzyme Commision number	3.1.3.11: fructose-bisphosphatase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	F6P	B	D2121 G2122 N2212 Y2215 Y2244 S2247 M2248 Y2264 K2274 E2280	D113 G114 N204 Y207 Y236 S239 M240 Y256 K266 E272
BS02	MG	B	E2097 D2118 D2121 E2280	E89 D110 D113 E272
BS03	PO4	B	E2097 D2118 D2121 G2122 S2123	E89 D110 D113 G114 S115
BS04	AMP	B	E2020 G2021 T2027 E2029 M2030 K2112 Y2113 R2140 V2160 M2177	E12 G13 T19 E21 M22 K104 Y105 R132 V152 M169	BindingDB: IC50=1300nM

Gene Ontology

	Molecular Function
GO:0016208	AMP binding
GO:0016787	hydrolase activity
GO:0016791	phosphatase activity
GO:0042132	fructose 1,6-bisphosphate 1-phosphatase activity
GO:0042578	phosphoric ester hydrolase activity
GO:0042802	identical protein binding
GO:0046872	metal ion binding
GO:0048029	monosaccharide binding

	Biological Process
GO:0005975	carbohydrate metabolic process
GO:0005986	sucrose biosynthetic process
GO:0006000	fructose metabolic process
GO:0006002	fructose 6-phosphate metabolic process
GO:0006094	gluconeogenesis
GO:0006111	regulation of gluconeogenesis
GO:0016311	dephosphorylation
GO:0030308	negative regulation of cell growth
GO:0030388	fructose 1,6-bisphosphate metabolic process
GO:0045820	negative regulation of glycolytic process
GO:0046580	negative regulation of Ras protein signal transduction
GO:0071286	cellular response to magnesium ion
GO:0071466	cellular response to xenobiotic stimulus

	Cellular Component
GO:0005737	cytoplasm
GO:0005829	cytosol

View graph for
Molecular Function

View graph for
Biological Process

View graph for
Cellular Component

External links

PDB	RCSB:1nv7, PDBe:1nv7, PDBj:1nv7
PDBsum	1nv7
PubMed	12595529
UniProt	P00636\|F16P1_PIG Fructose-1,6-bisphosphatase 1 (Gene Name=FBP1)

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