Structure of PDB 1njj Chain B

Receptor sequence

>1njjB (length=349) Species: 31285 (Trypanosoma brucei gambiense)

NTRDALCKKISGDPFFVADLGDIVRKHETWKKCLPRVTPFYAVKCNDDWR
VLGTLAALGTGFDCASNTEIQRVRGIGVPPEKIIYANPCKQISHIRYARD
SGVDVMTFDCVDELEKVAKTHPKAKMVLRISTVKFGAKVEDCRFILEQAK
KLNIDVTGVSFHVGSGSTDASTFAQAISDSRFVFDMGTELGFNMHILDIG
GGFPGTRDAPLKFEEIAGVINNALEKHFPPDLKLTIVAEPGRYYVASAFT
LAVNVIAKKVTQSFMYYVNDGVYGSFNCILYDHAVVRPLPQREPIPNEKL
YPSSVWGPTCDGLDQIVERYYLPEMQVGEWLLFEDMGAYTVSPTIYYVV

3D structure

• PDB: 1njj X-ray Structure Determination of Trypanosoma brucei Ornithine Decarboxylase Bound to D-Ornithine and to G418: INSIGHTS INTO SUBSTRATE BINDING AND ODC CONFORMATIONAL FLEXIBILITY.
• Chain: B
• Resolution: 2.45 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K69 H197 E274
• Catalytic site (residue number reindexed from 1): K44 H162 E239
• Enzyme Commision number: 4.1.1.17: ornithine decarboxylase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	GET	B	R22 R242 L339 P340 Q341 E343 E384 D385	R3 R207 L289 P290 Q291 E293 E334 D335	MOAD: Ki=8mM
BS02	ORX	B	K69 H197 G236 G237 E274 G276 R277 Y331 D332 Y389	K44 H162 G201 G202 E239 G241 R242 Y281 D282 Y339	MOAD: Kd=0.27mM

Gene Ontology

Molecular Function

• GO:0003824 catalytic activity
• GO:0004586 ornithine decarboxylase activity
• GO:0016829 lyase activity

Biological Process

• GO:0006596 polyamine biosynthetic process
• GO:0033387 putrescine biosynthetic process from ornithine

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1njj, PDBe:1njj, PDBj:1njj
• PDBsum: 1njj
• PubMed: 12672797
• UniProt: Q9TZZ6