Structure of PDB 1nir Chain B

Receptor sequence
>1nirB (length=539) Species: 287 (Pseudomonas aeruginosa) [Search protein sequence]
KAAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGVL
RKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITL
MAKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVT
LRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDM
IDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMD
GETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKV
LLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSK
DRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKN
HPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVF
DLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDS
SALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY
3D structure
PDB1nir N-terminal arm exchange is observed in the 2.15 A crystal structure of oxidized nitrite reductase from Pseudomonas aeruginosa.
ChainB
Resolution2.15 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) C47 C50 H51 M88 H327 H369
Catalytic site (residue number reindexed from 1) C43 C46 H47 M84 H323 H365
Enzyme Commision number 1.7.2.1: nitrite reductase (NO-forming).
1.7.99.1: hydroxylamine reductase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 HEC B C47 C50 H51 T59 L63 R71 L79 T84 L86 M88 P89 W91 C43 C46 H47 T55 L59 R67 L75 T80 L82 M84 P85 W87
BS02 DHE B R156 H182 I183 R185 R198 R225 S226 Y245 A283 A284 H327 R372 F425 F533 R152 H178 I179 R181 R194 R221 S222 Y241 A279 A280 H323 R368 F421 F529
Gene Ontology
Molecular Function
GO:0009055 electron transfer activity
GO:0016491 oxidoreductase activity
GO:0020037 heme binding
GO:0046872 metal ion binding
GO:0050418 hydroxylamine reductase activity
GO:0050421 nitrite reductase (NO-forming) activity
Cellular Component
GO:0042597 periplasmic space

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Molecular Function
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Cellular Component
External links
PDB RCSB:1nir, PDBe:1nir, PDBj:1nir
PDBsum1nir
PubMed9331415
UniProtP24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)

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