Structure of PDB 1n90 Chain B

Receptor sequence

>1n90B (length=539) Species: 287 (Pseudomonas aeruginosa)

KAAEQYQGAASAVDPAHVVRTNGAPDMSESEFNEAKQIYFQRCAGCHGVL
RKGATGKPLTPDITQQRGQQYLEALITYGTPLGMPNWGSSGELSKEQITL
MAKYIQHTPPQPPEWGMPEMRESWKVLVKPEDRPKKQLNDLDLPNLFSVT
LRDAGQIALVDGDSKKIVKVIDTGYAVHISRMSASGRYLLVIGRDARIDM
IDLWAKEPTKVAEIKIGIEARSVESSKFKGYEDRYTIAGAYWPPQFAIMD
GETLEPKQIVSTRGMTVDTQTYHPEPRVAAIIASHEHPEFIVNVKETGKV
LLVNYKDIDNLTVTSIGAAPFLHDGGWDSSHRYFMTAANNSNKVAVIDSK
DRRLSALVDVGKTPHPGRGANFVHPKYGPVWSTSHLGDGSISLIGTDPKN
HPQYAWKKVAELQGQGGGSLFIKTHPKSSHLYVDTTFNPDARISQSVAVF
DLKNLDAKYQVLPIAEWADLGEGAKRVVQPEYNKRGDEVWFSVWNGKNDS
SALVVVDDKTLKLKAVVKDPRLITPTGKFNVYNTQHDVY

3D structure

• PDB: 1n90 Does the reduction of c heme trigger the conformational change of crystalline nitrite reductase?
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): C47 C50 H51 M88 H327 H369
• Catalytic site (residue number reindexed from 1): C43 C46 H47 M84 H323 H365
• Enzyme Commision number: 1.7.2.1: nitrite reductase (NO-forming).
  1.7.99.1: hydroxylamine reductase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEC	B	C47 C50 H51 T59 L63 R71 L79 T84 L86 M88 P89 W91	C43 C46 H47 T55 L59 R67 L75 T80 L82 M84 P85 W87
BS02	DHE	B	R156 H182 I183 R185 R198 R225 S226 Y245 A283 A284 H327 R372 F425 W498 F533	R152 H178 I179 R181 R194 R221 S222 Y241 A279 A280 H323 R368 F421 W494 F529

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0016491 oxidoreductase activity
• GO:0020037 heme binding
• GO:0046872 metal ion binding
• GO:0050418 hydroxylamine reductase activity
• GO:0050421 nitrite reductase (NO-forming) activity

Cellular Component

• GO:0042597 periplasmic space

External links

• PDB: RCSB:1n90, PDBe:1n90, PDBj:1n90
• PDBsum: 1n90
• PubMed: 10329702
• UniProt: P24474|NIRS_PSEAE Nitrite reductase (Gene Name=nirS)