Structure of PDB 1ms0 Chain B

Receptor sequence

>1ms0B (length=623) Species: 5693 (Trypanosoma cruzi)

LAPGSSRVELFKRQSSKVPFEKDGKVTERVVHSFRLPALVNVDGVMVAIA
DARYETSFDNSLIDTVAKYSVDDGETWETQIAIKNSRASSVSRVVDPTVI
VKGNKLYVLVGSYNSSRSYWTSHGDARDWDILLAVGEVTKSTAGGKITAS
IKWGSPVSLKEFFPAEMEGMHTNQFLGGAGVAIVASNGNLVYPVQVTNKK
KQVFSKIFYSEDEGKTWKFGKGRSAFGCSEPVALEWEGKLIINTRVDYRR
RLVYESSDMGNTWLEAVGTLSRVWGPSPKSNQPGSQSSFTAVTIEGMRVM
LFTHPLNFKGRWLRDRLNLWLTDNQRIYNVGQVSIGDENSAYSSVLYKDD
KLYCLHEINSNEVYSLVFARLVGELRIIKSVLQSWKNWDSHLSSICTPAG
CGPAVTTVGLVGFLSHSATKTEWEDAYRCVNASTANAERVPNGLKFAGVG
GGALWPVSQQGQNQRYHFANHAFTLVASVTIHEVPKGASPLLGASLDSSG
GKKLLGLSYDKRHQWQPIYGSTPVTPTGSWEMGKRYHVVLTMANKIGSVY
IDGEPLEGSGQTVVPDERTPDISHFYVGGYKRSGMPTDSRVTVNNVLLYN
RQLNAEEIRTLFLSQDLIGTEAH

3D structure

• PDB: 1ms0 The crystal structure and mode of action of trans-sialidase, a key enzyme in Trypanosoma cruzi pathogenesis
• Chain: B
• Resolution: 2.5 Å

Enzymatic activity

• Enzyme Commision number: 3.2.1.18: exo-alpha-sialidase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	BGC	B	Y119 W312	Y119 W312
BS02	GAL	B	R35 D59 W312 E362	R35 D59 W312 E362
BS03	DAN	B	D59 D96 Q195 E230 R245 R314 Y342	D59 D96 Q195 E230 R245 R314 Y342	BindingDB: Ki=12300000nM

Gene Ontology

Molecular Function

• GO:0004308 exo-alpha-sialidase activity

Biological Process

• GO:0006689 ganglioside catabolic process
• GO:0009313 oligosaccharide catabolic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0016020 membrane
• GO:0043231 intracellular membrane-bounded organelle

External links

• PDB: RCSB:1ms0, PDBe:1ms0, PDBj:1ms0
• PDBsum: 1ms0
• PubMed: 12419220
• UniProt: Q26966