Structure of PDB 1mc1 Chain B

Receptor sequence
>1mc1B (length=498) Species: 1901 (Streptomyces clavuligerus) [Search protein sequence]
APVLPAAFGFLASARTGGGRAPGPVFATRGSHTDIDTPQGERSLAATLVH
APSVAPDRAVARSLTGAPTTAVLAGEIYNRDELLSVLPAGPAPEGDAELV
LRLLERYDLHAFRLVNGRFATVVRTGDRVLLATDHAGSVPLYTCVAPGEV
RASTEAKALAAHRDPKGFPLADARRVAGLTGVYQVPAGAVMDIDLGSGTA
VTHRTWTPGLSRRILPEGEAVAAVRAALEKAVAQRVTPGDTPLVVLSGGI
DSSGVAACAHRAAGELDTVSMGTDTSNEFREARAVVDHLRTRHREITIPT
TELLAQLPYAVWASESVDPDIIEYLLPLTALYRALDGPERRILTGYGADI
PLGGMHREDRLPALDTVLAHDMATFDGLNEMSPVLSTLAGHWTTHPYWDR
EVLDLLVSLEAGLKRRHGRDKWVLRAAMADALPAETVNRPKLTSSFSRLL
LDHGVAEDRVHEAKRQVVRELFDLTVGGGRHPSEVDTDDVVRSVADRT
3D structure
PDB1mc1 The catalytic cycle of beta -lactam synthetase observed by x-ray crystallographic snapshots
ChainB
Resolution2.16 Å
3D
structure
Catalytic site residues are labeled in the structure
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Enzymatic activity
Catalytic site (original residue number in PDB) A76 G77 D322 Y348 E382 K443
Catalytic site (residue number reindexed from 1) A74 G75 D320 Y346 E380 K441
Enzyme Commision number 6.3.3.4: (carboxyethyl)arginine beta-lactam-synthase.
Interaction with ligand
Site
#		 Ligand Ligand
chain		 Binding residues on receptor
(original residue number in PDB)		 Binding residues on receptor
(residue number reindexed from 1)		 Binding affinity
BS01 MG B D253 D351 D251 D349
BS02 PCX B Y326 Y348 G349 D351 I352 M357 D373 E382 K443 Y324 Y346 G347 D349 I350 M355 D371 E380 K441
BS03 AMP B V247 L248 S272 M273 L330 G347 Y348 V245 L246 S270 M271 L328 G345 Y346
BS04 POP B S249 G251 D253 S254 K423 K443 S247 G249 D251 S252 K421 K441
Gene Ontology
Molecular Function
GO:0004066 asparagine synthase (glutamine-hydrolyzing) activity
GO:0005524 ATP binding
GO:0016874 ligase activity
GO:0034027 (carboxyethyl)arginine beta-lactam-synthase activity
GO:0046872 metal ion binding
Biological Process
GO:0006529 asparagine biosynthetic process
GO:0033050 clavulanic acid biosynthetic process
Cellular Component
GO:0005829 cytosol

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Molecular Function
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Biological Process
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Cellular Component
External links
PDB RCSB:1mc1, PDBe:1mc1, PDBj:1mc1
PDBsum1mc1
PubMed12409610
UniProtP0DJQ7|BLS_STRCL Carboxyethyl-arginine beta-lactam-synthase (Gene Name=bls)

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