Structure of PDB 1m64 Chain B

Receptor sequence

>1m64B (length=568) Species: 56812 (Shewanella frigidimarina)

ADNLAEFHVQNQECDSCHTPDGELSNDSLTYENTQCVSCHGTLAEVAETT
KHEHYNAHASHFPGEVACTSCHSAHEKSMVYCDSCHSFDFNMPYAKKWLR
DEPTIAELAKDKSERQAALASAPHDTVDVVVVGSGGAGFSAAISATDSGA
KVILIEKEPVIGGNAKLAAGGMNAAWTDQQKAKKITDSPELMFEDTMKGG
QNINDPALVKVLSSHSKDSVDWMTAMGADLTDVGMMGGASVNRAHRPTGG
AGVGAHVVQVLYDNAVKRNIDLRMNTRGIEVLKDDKGTVKGILVKGMYKG
YYWVKADAVILATGGFAKNNERVAKLDPSLKGFISTNQPGAVGDGLDVAE
NAGGALKDMQYIFAHPTLSVKGGVMVTEAVRGNGAILVNREGKRFVNEIT
TRDKASAAILAQTGKSAYLIFDDSVRKSLSKIDKYIGLGVAPTADSLVKL
GKMEGIDGKALTETVARYNSLVSSGKDTDFERPNLPRALNEGNYYAIEVT
PGVHHTMGGVMIDTKAEVMNAKKQVIPGLYGAGEVTGGVHGANRLGGNAI
SDIITFGRLAGEEAAKYS

3D structure

• PDB: 1m64 Engineering water to act as an active site acid catalyst in a soluble fumarate reductase
• Chain: B
• Resolution: 1.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): M236 F363 H365 M375 E378 R381 R402 H504 H505 R544
• Catalytic site (residue number reindexed from 1): M236 F363 H365 M375 E378 R381 R402 H504 H505 R544
• Enzyme Commision number: 1.3.2.4: fumarate reductase (cytochrome).

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	HEM	B	V9 C14 C17 H18 L24 S73 A74 H75	V9 C14 C17 H18 L24 S73 A74 H75
BS02	HEM	B	L4 H8 Q12 C36 C39 H40 H72 Y94	L4 H8 Q12 C36 C39 H40 H72 Y94
BS03	HEM	B	H40 L43 H52 A57 H58 C68 C71 H72 F90 N91 M92	H40 L43 H52 A57 H58 C68 C71 H72 F90 N91 M92
BS04	HEM	B	S60 H61 F62 C82 C85 H86 V374 K431 Y435	S60 H61 F62 C82 C85 H86 V374 K431 Y435
BS05	FAD	B	V132 G135 A137 E156 K157 G163 N164 A165 L167 A168 A169 G170 G171 R277 G278 T313 Q338 D344 M375 H504 H505 E534 G547 A549 I550 I553	V132 G135 A137 E156 K157 G163 N164 A165 L167 A168 A169 G170 G171 R277 G278 T313 Q338 D344 M375 H504 H505 E534 G547 A549 I550 I553
BS06	FUM	B	G170 M375 T377 E378 H504 R544 G546	G170 M375 T377 E378 H504 R544 G546

Gene Ontology

Molecular Function

• GO:0009055 electron transfer activity
• GO:0010181 FMN binding
• GO:0016491 oxidoreductase activity
• GO:0046872 metal ion binding

Biological Process

• GO:0008202 steroid metabolic process
• GO:0009061 anaerobic respiration
• GO:0019645 anaerobic electron transport chain

Cellular Component

• GO:0030288 outer membrane-bounded periplasmic space
• GO:0042597 periplasmic space

External links

• PDB: RCSB:1m64, PDBe:1m64, PDBj:1m64
• PDBsum: 1m64
• PubMed: 12356299
• UniProt: P0C278|FCCA_SHEFR Fumarate reductase (cytochrome) (Gene Name=fccA)