Structure of PDB 1m54 Chain B

Receptor sequence

>1m54B (length=347) Species: 9606 (Homo sapiens)

RPDAPSRCTWQLGRPASESPHHHTAPAKSPKILPDILKKIGDTPMVRINK
IGKKFGLKCELLAKCEFFNAGGSVKDRISLRMIEDAERDGTLKPGDTIIE
PTSGNTGIGLALAAAVRGYRCIIVMPEKMSSEKVDVLRALGAEIVRTPTS
HVGVAWRLKNEIPNSHILDQYRNASNPLAHYDTTADEILQQCDGKLDMLV
ASVGTGGTITGIARKLKEKCPGCRIIGVDPEGSILAEPEELNQTEQTTYE
VEGIGYDFIPTVLDRTVVDKWFKSNDEEAFTFARMLIAQEGLLCGGSAGS
TVAVAVKAAQELQEGQRCVVILPDSVRNYMTKFLSDRWMLQKGFEED

3D structure

• PDB: 1m54 HUMAN CYSTATHIONINE BETA-SYNTHASE IS A HEME SENSOR PROTEIN. EVIDENCE THAT THE REDOX SENSOR IS HEME AND NOT THE VICINAL CYSTEINES IN THE CXXC MOTIF SEEN IN THE CRYSTAL STRUCTURE OF THE TRUNCATED ENZYME
• Chain: B
• Resolution: 2.9 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): K119 S147 D281 S285 L287 S349 P375
• Catalytic site (residue number reindexed from 1): K75 S103 D229 S233 L235 S297 P323
• Enzyme Commision number: 4.2.1.22: cystathionine beta-synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	PLP	B	K119 N149 G256 T257 G258 T260 E304 G305 S349 P375 D376	K75 N105 G204 T205 G206 T208 E252 G253 S297 P323 D324
BS02	HEM	B	S50 R51 C52 T53 W54 R58 E62 S63 P64 H65 A226 P229 L230 Y233 R266	S6 R7 C8 T9 W10 R14 E18 S19 P20 H21 A174 P177 L178 Y181 R214

Gene Ontology

Molecular Function

• GO:0004122 cystathionine beta-synthase activity

Biological Process

• GO:0006535 cysteine biosynthetic process from serine
• GO:0019343 cysteine biosynthetic process via cystathionine

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1m54, PDBe:1m54, PDBj:1m54
• PDBsum: 1m54
• PubMed: 12173932
• UniProt: P35520|CBS_HUMAN Cystathionine beta-synthase (Gene Name=CBS)