Structure of PDB 1lpf Chain B

Receptor sequence

>1lpfB (length=472) Species: 294 (Pseudomonas fluorescens)

SQKFDVVVIGAGPGGYVAAIRAAQLGLKTACIEKYIGKEGKVALGGTCLN
VGCIPSKALLDSSYKYHEAKEAFKVHGIEAKGVTIDVPAMVARKANIVKN
LTGGIATLFKANGVTSFEGHGKLLANKQVEVTGLDGKTQVLEAENVIIAS
GSRPVEIPPAPLSDDIIVDSTGALEFQAVPKKLGVIGAGVIGLELGSVWA
RLGAEVTVLEALDKFLPAADEQIAKEALKVLTKQGLNIRLGARVTASEVK
KKQVTVTFTDANGEQKETFDKLIVAVGRRPVTTDLLAADSGVTLDERGFI
YVDDHCKTSVPGVFAIGDVVRGAMLAHKASEEGVMVAERIAGHKAQMNYD
LIPSVIYTHPEIAWVGKTEQTLKAEGVEVNVGTFPFAASGRAMAANDTTG
LVKVIADAKTDRVLGVHVIGPSAAELVQQGAIGMEFGTSAEDLGMMVFSH
PTLSEALHEAALAVNGHAIHIA

3D structure

• PDB: 1lpf Three-dimensional structure of lipoamide dehydrogenase from Pseudomonas fluorescens at 2.8 A resolution. Analysis of redox and thermostability properties.
• Chain: B
• Resolution: 2.8 Å

Enzymatic activity

• Catalytic site (original residue number in PDB): P13 L44 C48 C53 S56 G82 V83 V190 E194 S330 F448 H450 E455
• Catalytic site (residue number reindexed from 1): P13 L44 C48 C53 S56 G82 V83 V190 E194 S330 F448 H450 E455
• Enzyme Commision number: 1.8.1.4: dihydrolipoyl dehydrogenase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	FAD	B	I9 G10 G12 P13 E33 T47 C48 G52 C53 K57 H120 G121 A149 S150 G151 S170 I191 R278 D318 M324 L325 A326	I9 G10 G12 P13 E33 T47 C48 G52 C53 K57 H120 G121 A149 S150 G151 S170 I191 R278 D318 M324 L325 A326

Gene Ontology

Molecular Function

• GO:0004148 dihydrolipoyl dehydrogenase activity
• GO:0016491 oxidoreductase activity
• GO:0016668 oxidoreductase activity, acting on a sulfur group of donors, NAD(P) as acceptor
• GO:0050660 flavin adenine dinucleotide binding

Cellular Component

• GO:0005737 cytoplasm

External links

• PDB: RCSB:1lpf, PDBe:1lpf, PDBj:1lpf
• PDBsum: 1lpf
• PubMed: 8487301
• UniProt: P14218|DLDH_PSEFL Dihydrolipoyl dehydrogenase (Gene Name=lpd)