Structure of PDB 1kph Chain B

Receptor sequence

>1kphB (length=285) Species: 1773 (Mycobacterium tuberculosis)

DELKPHFANVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAK
IDLALGKLGLQPGMTLLDVGCGWGATMMRAVEKYDVNVVGLTLSKNQANH
VQQLVANSENLRSKRVLLAGWEQFDEPVDRIVSIGAFEHFGHERYDAFFS
LAHRLLPADGVMLLHTITGLHPKEIHERGLPMSFTFARFLKFIVTEIFPG
GRLPSIPMVQECASANGFTVTRVQSLQPHYAKTLDLWSAALQANKGQAIA
LQSEEVYERYMKYLTGCAEMFRIGYIDVNQFTCQK

3D structure

• PDB: 1kph Crystal structures of mycolic acid cyclopropane synthases from Mycobacterium tuberculosis
• Chain: B
• Resolution: 2.0 Å

Enzymatic activity

• Enzyme Commision number: 2.1.1.79: cyclopropane-fatty-acyl-phospholipid synthase.

Interaction with ligand

Site #	Ligand	Ligand chain	Binding residues on receptor (original residue number in PDB)	Binding residues on receptor (residue number reindexed from 1)	Binding affinity
BS01	CO3	B	S34 C35 H167 I169 Y232	S32 C33 H165 I167 Y230
BS02	SAH	B	V12 Y16 T32 S34 G72 G74 T94 L95 Q99 G122 W123 E124 I136 A138 H141 F142	V10 Y14 T30 S32 G70 G72 T92 L93 Q97 G120 W121 E122 I134 A136 H139 F140
BS03	10A	B	Y16 Y33 G137 I169 L192 F200 G203 L205 Y232 Y265 C269 I278	Y14 Y31 G135 I167 L190 F198 G201 L203 Y230 Y263 C267 I276

Gene Ontology

Molecular Function

• GO:0008168 methyltransferase activity
• GO:0008825 cyclopropane-fatty-acyl-phospholipid synthase activity

Biological Process

• GO:0006629 lipid metabolic process
• GO:0008610 lipid biosynthetic process
• GO:0032259 methylation
• GO:0046500 S-adenosylmethionine metabolic process
• GO:0071768 mycolic acid biosynthetic process

Cellular Component

• GO:0005737 cytoplasm
• GO:0005886 plasma membrane

External links

• PDB: RCSB:1kph, PDBe:1kph, PDBj:1kph
• PDBsum: 1kph
• PubMed: 11756461
• UniProt: P9WPB7|CMAS1_MYCTU Cyclopropane mycolic acid synthase 1 (Gene Name=cmaA1)